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We narrowed to 32,749 results for: CMV

Showing: 5561 - 5580 of 32749 results

  1. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  2. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

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  3. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199368
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOstar-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199368-plasmid-map-sequence-id-389900

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  5. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

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  6. pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA

    Plasmid
    #246432
    Purpose
    All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain
    mCherry
    ωRNA
    Tags
    HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
    Expression
    Mammalian
    Mutation
    R-IscB: changed Aspartic Acid 60 to Alanine, chan…
    Promoter
    CMV and U6
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    246432-plasmid-map-sequence-id-490908

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  7. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid
    #241142
    Purpose
    Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-HA frankenbody
    tdMCP
    tdPCP
    Tags
    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    241142-plasmid-map-sequence-id-487015

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  14. JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF

    Plasmid
    #236153
    Purpose
    Express mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    SCN1A-377-deImmLink-NZF
    Expression
    Mammalian
    Mutation
    G2E, A104K, S109I in SCN1A-377
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236153-plasmid-map-sequence-id-468410

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  18. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid
    #228400
    Purpose
    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    NES/PKI-mNeonGreen2x-NLS/FUS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228400-plasmid-map-sequence-id-457259

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Showing: 5561 - 5580 of 32749 results