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AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-HyPerFLEX-UnaG-WPRE

Plasmid

#217564

Purpose

Expression of HyPerFLEX fused with UnaG in mammalian neurons

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-HyPerFLEX-UnaG-WPRE

Plasmid

#217565

Purpose

Mammalian expression of HyPerFLEX fused with UnaG

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NES-WPRE

Plasmid

#217562

Purpose

Mammalian expression of HyPerFLEX targeted to the cytoplasm

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NES

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HyPerFLEX-Sec61b

Plasmid

#238940

Purpose

Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Sec61b

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC12

Plasmid

#205068

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Expression

Bacterial

Mutation

Lacks C-terminal 12 amino acids

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC16

Plasmid

#205069

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Expression

Bacterial

Mutation

Lacks C-terminal 16 amino acids

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC18

Plasmid

#205070

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Expression

Bacterial

Mutation

Lacks C-terminal 18 amino acids

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC20

Plasmid

#205071

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Expression

Bacterial

Mutation

Lacks C-terminal 20 amino acids

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC9

Plasmid

#205067

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Expression

Bacterial

Mutation

Lacks C-terminal 9 amino acids

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/sh-FYN1-GFP_Seq_C

Plasmid

#201399

Purpose

Knockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRV∆G-N*PEST-4Cre

Plasmid

#174381

Purpose

Rabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

Cre recombinase

Expression

Mammalian

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

Plasmid

#162965

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

Plasmid

#162964

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

Plasmid

#162957

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

Plasmid

#162955

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

Plasmid

#162954

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166986

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166991

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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