We narrowed to 22,509 results for: cas9

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pvPE-V4

Plasmid

#240288

Purpose

Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

Depositor

Lusheng Huang

Publication

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Insert

pvPE-V4

Use

CRISPR

Tags

SV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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TS-PE2-N

Plasmid

#176649

Purpose

Expresses N-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Publication

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

N-terminus of PE2
Artificial SD

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and None

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

Plasmid

#69226

Purpose

Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

Plasmid

#120293

Purpose

AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

N-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

SV40-NLS and split-intein

Expression

Mammalian

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Publication

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Publication

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

Plasmid

#138295

Purpose

gRNA for CRISPR/Cas9 knockout of human TRIM21

Depositor

Gaudenz Danuser

Publication

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

gRNA TRIM21 (TRIM21 Human)

Use

CRISPR

Promoter

U6

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Publication

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9GFP-W

Plasmid

#200100

Purpose

Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

CRISPR and Lentiviral

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Publication

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Publication

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Publication

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Publication

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-St1Cas9-BlastR

Plasmid

#220976

Purpose

Expresses human codon-optimized St1Cas9

Depositor

Hyongbum Kim

Publication

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

St1Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SlugCas9-HF-BlastR

Plasmid

#220973

Purpose

Expresses human codon-optimized SlugCas9-HF

Depositor

Hyongbum Kim

Publication

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9-HF

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Sa-SlugCas9-BlastR

Plasmid

#220968

Purpose

Expresses human codon-optimized Sa-SlugCas9

Depositor

Hyongbum Kim

Publication

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Sa-SlugCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pX330 C-FKBP-Cas9

Plasmid

#162729

Purpose

Mammalian expression

Depositor

Amit Choudhary

Publication

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HeFm1SpCas9

Plasmid

#92110

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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