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pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

Plasmid

#83489

Purpose

cre-dependent PAK1-AID under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

PAK1-AID-ires-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Ad-Flag-HDAC3

Plasmid

#63675

Purpose

adenoviral expression of Flag tagged HDAC3

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mammalian

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 1-590

Plasmid

#11624

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
PPP2R2B B6.5 gRNA

Plasmid

#90848

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2B (Guide Designation B6.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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LIN52 D6.4 gRNA

Plasmid

#90735

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIN52

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LIN52 (Guide Designation D6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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PLK1 G5.1 gRNA

Plasmid

#90834

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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NAE1 G7.3 gRNA

Plasmid

#90776

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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DEP-GFP

Plasmid

#109043

Purpose

Expression of the Dishevelled DEP domain

Depositor

Mariann Bienz

Article

Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

Insert

dishevelled segment polarity protein 2 (DVL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Dvl2 DEP domain

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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PSMD14 E6.4 gRNA

Plasmid

#90859

Purpose

3rd generation lentiviral gRNA plasmid targeting human PSMD14

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PSMD14 (Guide Designation E6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CFP-OSM

Plasmid

#12190

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 1-332

Plasmid

#11625

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino-terminal half of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
ATAD2 D7.3 gRNA

Plasmid

#90538

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATAD2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ATAD2 (Guide Designation D7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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PLK1 G6.1 gRNA

Plasmid

#90835

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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CEP55 B12.2 gRNA

Plasmid

#90623

Purpose

3rd generation lentiviral gRNA plasmid targeting human CEP55

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CEP55 (Guide Designation B12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Nov. 15, 2017

Availability

Academic Institutions and Nonprofits only

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CEP55 B11.2 gRNA

Plasmid

#90622

Purpose

3rd generation lentiviral gRNA plasmid targeting human CEP55

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CEP55 (Guide Designation B11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

Plasmid

#87917

Purpose

Gateway cloning

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgHTT1, sgGFP2, shCas9

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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