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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,895 results for: nls

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Showing: 5561 - 5580 of 9895 results

  3. pNSEN-d2

    Plasmid
    #59763
    Purpose
    Expresses nuclear EGFP and Tomato in mammalian cells
    Depositor
    Jerry Crabtree
    Publication
    Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    d2-EGFP
    Tomato
    Tags
    3X NLS and PEST
    Expression
    Mammalian
    Promoter
    CMV promoter and SV40 promoter
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    59763-plasmid-map-sequence-id-94069

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  9. pCSD_FRB-TALETS3-3xFLAG-2xNLS

    Plasmid
    #107306
    Purpose
    Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    TALE_VEGFA-TS3
    Use
    CRISPR
    Tags
    3x Flag, 2xNLS and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107306-plasmid-map-sequence-id-204787

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  10. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98998-plasmid-map-sequence-id-190366

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  11. pcDNA3.1-CibN-dCas9

    Plasmid
    #60551
    Purpose
    Expresses CibN-dCas9 in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CibN-dCas9
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60551-plasmid-map-sequence-id-97050

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  15. pDD3248 pCS2-mtagBFP2-p2a-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

    Plasmid
    #251770
    Purpose
    Blue fluorescent protein-tagged TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay
    Depositor
    Dave Dingal
    Publication
    Lim Suan et al Nat Commun 17, 3257 (2026).
    Insert
    arrestin, beta 2a (arrb2a Zebrafish)
    Tags
    NLS (SV40), sfCherry3C large fragment and mTagBFP…
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    251770-plasmid-map-sequence-id-504794

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  16. pGfa2-nLac

    Plasmid
    #53126
    Purpose
    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.
    Depositor
    Michael Brenner
    Publication
    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    human GFAP
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    53126-plasmid-map-sequence-id-82755

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  17. pHS1517

    Plasmid
    #205980
    Purpose
    FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas5-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205980-plasmid-map-sequence-id-416244

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  18. pHS1862

    Plasmid
    #205975
    Purpose
    FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas5 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205975-plasmid-map-sequence-id-416239

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  19. pHS1859

    Plasmid
    #205972
    Purpose
    FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205972-plasmid-map-sequence-id-416242

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  20. pHS1860

    Plasmid
    #205973
    Purpose
    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205973-plasmid-map-sequence-id-416241

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Showing: 5561 - 5580 of 9895 results