We narrowed to 2,757 results for: fas
-
Plasmid#198667PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H01L
Plasmid#198665PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertX3_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1
Plasmid#181812PurposeExpresses human MUC2 D1, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoGFP
Plasmid#44385DepositorInsertmitoGFP (COX8A Human)
UseLentiviralTagsCox8 targeting sequence and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive native promotersAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEND-193_Cacnes_HS
Plasmid#225622PurposeC. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)DepositorInsertHspR
UseSynthetic BiologyTagspFAST-6XHisExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA1
Plasmid#25868DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEND-192_Cacnes_HS
Plasmid#225621PurposeC. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).DepositorInsertHspR
UseSynthetic BiologyTagspFAST-6XHisExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA2
Plasmid#25869DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterUser-definedAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-NT-3/D15A
Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralTagsExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-mini
Plasmid#196338PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1 with cleavable His tag
Plasmid#181813PurposeExpresses human MUC2 D1 with cleavable His tag, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
UseTags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f
Plasmid#162373PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8f
UseTags6xHisExpressionMammalianMutationQ315LPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8s
Plasmid#162371PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8s
UseTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
UseTagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag
Plasmid#187994PurposeExpresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cellsDepositorInsertmucin2 D1D2D'D3 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D' with cleavable His tag
Plasmid#187993PurposeExpresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cellsDepositorInsertMucin2 D1D2D' (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p1585588
Plasmid#62620PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1_mouseST6galI
Plasmid#178435Purposemurine St6gal1 expression plasmidDepositorInsertSt6gal1 (St6gal1 Mouse)
UseTags6xHIS-TEV Cleavage SiteExpressionMammalianMutationCoding sequnce for amino acids 85-403PromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10
Plasmid#162386PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10
Plasmid#162388PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10
Plasmid#162383PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
Plasmid#162385PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
Plasmid#162387PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEXneo-TrkB
Plasmid#190203PurposeTo express the Human TrkB receptor under the Moloney mouse sarcoma virus LTRDepositorInsertTrkB (NTRK2 Human)
UseTagsExpressionMammalianMutationPromoterMoloney mouse sarcoma virus right LTRAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mEmerald Rab7a
Plasmid#115238PurposeTo express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.DepositorInsertRab7a fused to mEmerald (RAB7A Human)
UseAAVTagsmEmeraldExpressionMammalianMutation7 Amino Acids (21 Nucleotides) between mEmerald a…PromoterHuman UbCAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only