We narrowed to 21,206 results for: ACE

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pQmod3S-GG

Plasmid

#191349

Purpose

Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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BL21 Rosetta2 ΔrecBCD GamS

Bacterial Strain

#176586

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecB GamS

Bacterial Strain

#176585

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(+36)-DL4

Plasmid

#199168

Purpose

used to express a supercharged GFP variant that forms inclusion bodies in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)-DL4

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK005-3a-αMF

Plasmid

#84964

Purpose

Type 3a, α-mating factor - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK020-7-attB

Plasmid

#84989

Purpose

Type 7, BxbI recognition site

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

BxbI recognition site

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1711 t to a)

Promoter

Non

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK017-3b-yEGFP_Sec

Plasmid

#84976

Purpose

Type 3b, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK015-3-yEGFP_Intra

Plasmid

#84974

Purpose

Type 3, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK019-4-tAOX1

Plasmid

#84988

Purpose

Type 4, tAOX, Alcohol oxidase 1 terminator

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 terminator

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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sTR056

Plasmid

#177369

Purpose

Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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ZP30 Fgf8

Plasmid

#16912

Depositor

Randall Moon

Insert

fgf8 (fgf8a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pPTK004-2-pTPI1

Plasmid

#84963

Purpose

Type 2, pTPI1, Triose phosphate isomerase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Triose phosphate isomerase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK009-3a-αAmylase-αMFΔ

Plasmid

#84968

Purpose

Type 3a, α-Amylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-Amylase followed by MFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK010-3a-Glucoamylase-αMFΔ

Plasmid

#84969

Purpose

Type 3a, Glucoamylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glucoamylase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK006-3a-αMF no EAEA

Plasmid

#84965

Purpose

Type 3a, α-mating factor no EAEA - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no EAEA secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK018-3b-RFP_Sec

Plasmid

#84977

Purpose

Type 3b, Red fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1677 c to g and 1794 a to g)

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK001-2-pAOX1

Plasmid

#84960

Purpose

Type 2, pAOX1, Alcohol oxidase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK013-3a-Invertase-αMFΔ

Plasmid

#84972

Purpose

Type 3a, Invertase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Invertase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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