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Showing: 541 - 560 of 28674 results

  2. BcatMutS33/S37.T41/S45

    Plasmid
    #24204
    Depositor
    David Rimm
    Insert
    beta catenin (CTNNB1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S33A, S37A, T41A,S45A
    Promoter
    CMV
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only
    24204-plasmid-map-sequence-id-235877

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  6. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid
    #154196
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC1-A, sgRNA-BC1-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154196-plasmid-map-sequence-id-308676

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  7. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  8. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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  11. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

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  18. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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Showing: 541 - 560 of 28674 results