Addgene

We narrowed to 1,837 results for: IRES-GFP

Showing: 541 - 560 of 1837 results

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pMSCV-IRES-GFP-MD2 (F126A)-HA

Plasmid

#133067

Purpose

Retroviral expression of mouse MD2 (F126A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (F126A) (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

F126A

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (C95A)-HA

Plasmid

#133066

Purpose

Retroviral expression of mouse MD2 (C95A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (C95A) (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C95A

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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MRLC-DD-pIRES-EGFP-AP4-mito

Plasmid

#101287

Purpose

To define the role of Mena/VASP in tension-sensitive actin assembly

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

MRLC-DD and AP4-Mito

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-shift-IRES-GFP(KaganE21)

Plasmid

#52004

Purpose

Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-Shift

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

A 2 nucleotide insertion was introduced between t…

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Use

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS puro-GFP-BamHI-IRES-mCherry

Plasmid

#232247

Purpose

AcGFP1-BamHI-IRES-mCherry empty backbone

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

Plasmid

#133027

Purpose

Expresses EGFP-tagged late endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB7A (RAB7A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Tags

Expression

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJRH-1364 LV EF1a-CD4 IRES-EGFP

Plasmid

#201920

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD4 (CD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-gate-FLAG-IRES-GFP

Plasmid

#107398

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-flag-myr-Akt1-IRES-GFP

Plasmid

#65063

Purpose

mammalian retroviral expression plasmid

Depositor

Kira Gritsman, Jean Zhao

Article

Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

Insert

Akt1 (AKT1 Human)

Use

Tags

flag and myristoylation tag

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Use

Tags

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

Plasmid

#134867

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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