We narrowed to 9,220 results for: Pol

1. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

   Plasmid

   #193968

   Purpose

   Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   hPol eta (POLH Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-TgKI-MCS-eGFP-MCS-polyA

   Plasmid

   #174024

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-EGFP barcode-1-SV40 polyA

   Plasmid

   #190865

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode1
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-EGFP barcode-0-SV40 polyA

   Plasmid

   #190864

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode0
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-EGFP barcode-3-SV40 polyA

   Plasmid

   #190867

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode3
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-EGFP barcode-4-SV40 polyA

   Plasmid

   #190868

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode4
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-EGFP barcode-2-SV40 polyA

   Plasmid

   #190866

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode2
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

   Plasmid

   #84604

   Purpose

   Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

   Depositor

   James Chen

   Article

   Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

   Insert

   mCherry
   

   Use

   Zebrafish transgenesis

   Promoter

   8xGliBS-delta-crystallin minimal promoter

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

   Plasmid

   #218868

   Purpose

   Mammalian expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   CAG

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

    Plasmid

    #154004

    Purpose

    Express CasRx

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    CRISPR

    Promoter

    CAG

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

    Plasmid

    #232935

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Grb10 homology arms
    

    Use

    Mouse Targeting

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-TgKI-MCS-p2A-tdTomato-polyA

    Plasmid

    #174027

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-tdTomato
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-TgKI-MCS-mScarlet-MCS-polyA

    Plasmid

    #174023

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mScarlet
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. single polypeptide FPX biosensor for both caspase-3 and -8

    Plasmid

    #60886

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3 and 8
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-Flag-Polß(D256A)-IRES-puro

    Plasmid

    #128677

    Purpose

    mammalian expression of Flag-Polß(D256A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D256A

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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