Addgene

We narrowed to 1,222 results for: TIR

Showing: 541 - 560 of 1222 results

Results per page:

Sort results by:

pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

Plasmid

#192616

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RIboL1-jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

Plasmid

#192618

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hCD4-mOrange

Plasmid

#110192

Purpose

Encodes for human CD4 coding sequence tagged with the mOrange FP

Depositor

Sergi Padilla Parra

Article

Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

Insert

hCD4 (CD4 Human)

Use

Tags

mOrange

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise-parkin

Plasmid

#110945

Purpose

Expresses the ubiquitin ligase parkin fused to mTurquoise

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

E3 ubiquitin ligase parkin (PRKN Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
7749 Bicistronic_ires_hygro

Plasmid

#64374

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
8200 Bicistronic_GFP_ires_hygro

Plasmid

#64375

Purpose

This a retroviral expression plasmid expressing GFP along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

EGFP

Use

Retroviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-Venus-Akaluc (neo)

Plasmid

#124701

Purpose

Aka-Luciferase reporter

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Akaluciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hPGK

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VARP

Plasmid

#42312

Depositor

Thierry Galli

Article

Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

Insert

VARP (ANKRD27 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge