Addgene

We narrowed to 6,130 results for: WUS;

Showing: 541 - 560 of 6130 results

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-N152S-HA

Plasmid

#198182

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) N152S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The asparagine in IRG1 at position 152 mutates to…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-L272Q-HA

Plasmid

#198184

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The leucine in IRG1 at position 272 mutates to gl…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-VPS35

Plasmid

#21689

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

last nucleotide of stop codon removed for c-termi…

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn (human synapsin)

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-UDP1.0-IRES-mCherry-CAAX

Plasmid

#220850

Purpose

CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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Fuw-AcrIIA4-P2A-GFP

Plasmid

#108247

Purpose

Lentiviral vector to express AcrIIA4-P2A-GFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

AcrIIA4-NLS-HA (NEWENTRY Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C88S-FLAG

Plasmid

#198187

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C88S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA-CGG

Plasmid

#108248

Purpose

Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

sgRNA targeting GGCGGCGGCGGCGGCGGCGG

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAMKIIa

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H159Q-HA

Plasmid

#198183

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 159 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSin-NE1m

Plasmid

#172883

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

Depositor

J. Julius Zhu

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Insert

NE SENSOR

Use

Tags

Expression

Mammalian

Mutation

Promoter

sp6

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H277Y-HA

Plasmid

#198185

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 277 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Plasmid

#52985

Purpose

split Venus attached to MCP and PCP respectively

Depositor

Robert Singer

Article

Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

Insert

nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

ubc

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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phage-ubc-nls-ha-tdPCP-gfp

Plasmid

#40650

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

tdPCP-gfp

Use

Lentiviral

Tags

EGFP, HA, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C (UBC)

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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LsgRNA-MS2

Plasmid

#235597

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-MVPH

Plasmid

#235600

Purpose

Expresses the MS2-VP64-P65-HSF1 fusion protein

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

MS2-VP64-P65-HSF1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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