We narrowed to 12,262 results for: cel.2

1. pCMV-SARS-CoV-2 Nsp3TMC111A

   Plasmid

   #204207

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   Cys to Ala in active site of PLpro

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK-2-TGFBR2-m3_3'UTR

   Plasmid

   #31883

   Depositor

   Robert Blelloch

   Article

   Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

   Insert

   TGFBR2 mutant 3'UTR (TGFBR2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Third miR-302b, 372 7-mer seed match mutated from…

   Promoter

   SV40

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

3. GFP-Bcl-2-K17R-K22R

   Plasmid

   #220294

   Purpose

   mammalian expression of Bcl-2-K17R-K22R tagged with GFP

   Depositor

   Xinlai Cheng

   Article

   Wang et al Cell Reports Physical Science, Volume 5,

   Insert

   Bcl2 (BCL2 Human)
   

   Tags

   Emerald GFP

   Expression

   Mammalian

   Mutation

   K17R-K22R

   Promoter

   CMV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-SARS-CoV-2-3CL

   Plasmid

   #160278

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. kiCAP-AAV-BI-hTFR1-2

   Plasmid

   #218797

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1-2
   

   Expression

   Mammalian

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXL003-ishRNA-beta-catenin-2

   Plasmid

   #36299

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-2 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Promoter

   TetO-H1

   Available Since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

7. ABHD2-3'UTR-Del-2

   Plasmid

   #190096

   Purpose

   examine the activity of ABHD2 3'UTR

   Depositor

   Bowen Liu

   Article

   Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

   Insert

   ABHD2 3' UTR delete-2 (ABHD2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   deleted the base pairs 7122-7129 refer to NM_0070…

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. INCTbiosyn-pEF-GFP(rc)2

   Plasmid

   #127504

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 161+80pCalb2/mutCREB(-2)-like

   Plasmid

   #66751

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, mutant)

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp +80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   GCGC (-2) to TAGA

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAL4-DBD-CEBPa-AroPERFECT_IS15+2

    Plasmid

    #215605

    Purpose

    For overexpression of Gal4-DBD CEBPa AroPERFECT IS15+2

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa AroPERFECT IS15+2 (CEBPA Human)
    

    Expression

    Mammalian

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAL4-DBD-HOXD4-AroPERFECT-2

    Plasmid

    #215576

    Purpose

    For overexpression of Gal4-DBD HOXD4 AroPERFECT-2

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    HOXD4 AroPERFECT-2 (HOXD4 Human)
    

    Expression

    Mammalian

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. INCTbiosyn-p35S-GFP(rc)2

    Plasmid

    #127521

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX SHP-2(NC)-SH2

    Plasmid

    #46499

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(NC) (PTPN11 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-222

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX SHP-2(N)-SH2

    Plasmid

    #46498

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(N) (PTPN11 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-104

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX SHP-2(C)-SH2

    Plasmid

    #46497

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(C) (PTPN11 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 101-222

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIB_GST-nsp13 (SARS-CoV-2)

    Plasmid

    #169190

    Purpose

    Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    GST

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-cell-mCer

    Plasmid

    #185601

    Purpose

    Lentiviral construct for expression of mCerulean

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    cell-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-cell-tagRFP

    Plasmid

    #185598

    Purpose

    Lentiviral construct for expression of tagRFP-T

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    cell-tagRFP-T
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-cell-mEm

    Plasmid

    #185595

    Purpose

    Lentiviral construct for expression of mEmerald

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    cell-mEm
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Beta-2-adrenergic receptor-YFP

    Plasmid

    #67146

    Purpose

    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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