We narrowed to 2,494 results for: pcas9

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PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

Plasmid

#107858

Purpose

To knock-out TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

gRNA against TARDBP (Exon 2) (TARDBP Human)

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS

Plasmid

#69220

Purpose

Expresses wild type SpCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-wtSpCas9 (without sgRNA)

Plasmid

#92353

Purpose

Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-wild-type SpCas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-Intergenic sgRNA

Plasmid

#237463

Purpose

All-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun 17, 1218 (2026)

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-Intergenic sgRNA

Plasmid

#237464

Purpose

All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun 17, 1218 (2026)

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237466

Purpose

All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun 17, 1218 (2026)

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237467

Purpose

All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun 17, 1218 (2026)

Insert

sgRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

Plasmid

#231998

Purpose

Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B-targeted sgRNA (CHMP2B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

Plasmid

#210391

Purpose

AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

Depositor

Benjamin Kleinstiver

Article

Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

Insert

AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CamKII promoter for Cre, human U6 promoter for Sp…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

Plasmid

#223111

Purpose

pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-P2A-EGFP (MSP2582)

Plasmid

#223067

Purpose

pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized SpCas9-P2A-EGFP

Use

CRISPR

Tags

NLS(SV40)-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-BRD4-HaloTag-Guide-hspCas9

Plasmid

#228576

Purpose

Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

Depositor

Marcin Imielinski

Insert

BRD4 gRNA (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human Rab7A (RAB7A Human)

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

Plasmid

#220494

Purpose

To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting LIG4 exon 3 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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