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Plasmid#105281PurposeOverexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.DepositorInsertRcy1 (RCY1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGFH14A6-12
Plasmid#39543DepositorInsertSRP14 (SRP14 Human)
UseTagsEGFPExpressionMammalianMutationdeleted amino acids 101-113PromoterCMVAvailable sinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
SEAP (Protein secretion reporter) in pLX317
Plasmid#233290PurposeSEAP reporter (used for monitoring protein secretion)DepositorInsertSEAP
UseLentiviralTagsExpressionMammalianMutationPromoterE1FaAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2065
Plasmid#162150PurposeExpression plasmid for MlaE Trp149Bpa mutant for in vivo crosslinkingDepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-(6xHis-MlaE, Trp149Bpa)-MlaD-MlaC-MlaBPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB73
Plasmid#185437PurposeNUP1 CEN URA3 vector for synthetic lethal sectoring assays and screeningDepositorInsertNUP1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationshorter branch to 3' splice site distancePromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAL3519
Plasmid#47238DepositorInsertZebrafishCommunity-prp19-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3518
Plasmid#47237DepositorInsertZebrafishCommunity-prp19-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-hygro-mCherry-RAMP4
Plasmid#118391Purposestable lentiviral expression of cDNADepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX307 RFP
Plasmid#163976PurposeMammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)DepositorInsertRFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIVt
Plasmid#81092PurposeSingle Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-T
Plasmid#51232Purposebi-directional expression vector for yeast, constitutively active, TRP1 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPD/ADH1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
UseTagsExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
YEp112-CUP1-His-Smt3
Plasmid#99537PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
Plasmid#140153PurposeExpresses a regulatable vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo
Plasmid#115420PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG424-10X-tRNA
Plasmid#70125PurposeYeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae intronsDepositorInsert10X-tRNA block
UseTagsExpressionYeastMutationPromoterNone – SUP4 pol III promoter provided by insertAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG424-ccdB
Plasmid#70124PurposeYeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterNoneAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp204-P30-His-POL30
Plasmid#99550PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only