We narrowed to 9,744 results for: try

1. pJExpress414_GmPOPB-W695del

   Plasmid

   #92241

   Purpose

   Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Tryptophan 695 deleted

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPOTv7-puro-OsAID (p5109)

   Plasmid

   #239359

   Purpose

   pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

   Depositor

   Mark Carrington

   Article

   Alexandra et al bioRxiv 2025.06.17.660065

   Insert

   Rice Auxin Inducible Degradation domain
   

   Use

   Bacterial

   Promoter

   none

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrex-n-eGFP

   Plasmid

   #62544

   Purpose

   Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   eGFP
   

   Use

   (1)trypanosoma cruzi expression of egfp (2) genom…

   Tags

   None

   Mutation

   None

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. WasCFP /pQE-30

   Plasmid

   #79858

   Purpose

   Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

   Insert

   WasCFP
   

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Cyan fluorescent protein mCerulean with substitut…

   Promoter

   T5 promoter / lac operator

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrex-Luc-Neo

   Plasmid

   #48336

   Purpose

   Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

   Depositor

   Rick Tarleton

   Article

   Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

   Insert

   firely luciferase
   

   Use

   Trypanasoma cruzi expression

   Promoter

   T. cruzi rRNA

   Available Since

   July 20, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pYZ033

   Plasmid

   #98404

   Purpose

   Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIIS18-BsaI

   Plasmid

   #128649

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrex-b-NLS-hSpCas9

   Plasmid

   #62543

   Purpose

   Expression of Cas9 in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   NLS-hSpCas9
   

   Use

   CRISPR; Trypanosoma cruzi expression

   Tags

   3xFLAG and NLS

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CALCOCO1 W47A-mycDDK

   Plasmid

   #137769

   Purpose

   CALCOCO1 LIR mutant

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 W47A
   

   Tags

   mycDDK

   Expression

   Mammalian

   Mutation

   W47A

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRPa-iSL(MCS1+2)

    Plasmid

    #69244

    Purpose

    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrex-Luc-Phleo

    Plasmid

    #48337

    Purpose

    Trasgenically expresses firefly luciferase in T. cruzi with phleomycin selection

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    firely luciferase
    

    Use

    Trypanasoma cruzi expression

    Promoter

    T. cruzi rRNA

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pIIS18-SapI

    Plasmid

    #128599

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIIS18-BsmBI

    Plasmid

    #128648

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11a-DAAT-V33G/T242G

    Plasmid

    #159278

    Purpose

    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

    Depositor

    Roberto Chica

    Article

    Parmeggiani et al ACS Catalysis 9, 3482–3486.

    Insert

    Engineered d-alanine aminotransferase
    

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Valine 33 and threonine 242 have been mutated to …

    Promoter

    T7

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CALCOCO1 V142A-mycDDK

    Plasmid

    #137768

    Purpose

    CALCOCO1 CLIR mutant

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 V142A
    

    Tags

    mycDDK

    Expression

    Mammalian

    Mutation

    V142A

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 LC3B2 G120

    Plasmid

    #123077

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 G120

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 LC3B2 G120A

    Plasmid

    #123076

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 G120A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL2084

    Plasmid

    #137726

    Purpose

    Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

    Depositor

    Jeremy Mottram

    Article

    Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

    Type

    Empty backbone

    Use

    RNAi; Trypanosoma brucei tet inducible stem loop

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPTI-21

    Plasmid

    #195058

    Purpose

    simplyfied amino acid sequence by alanine scanning

    Depositor

    Peter Kim, Yutaka Kuroda

    Article

    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

    Insert

    bovine pancreatic trypsin inhibitor
    

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    21 amino acids are Alanine out of 58 amino acids

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPQD0CM0765

    Plasmid

    #177031

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    tryptophan decarboxylase
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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