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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

Promoter

EF1α1.1

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL649 CHYRON2 integration construct

Plasmid

#126446

Purpose

To integrate the CHYRON2 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON2 hgRNA
pCMV-puro

Use

Tags

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ14256

Plasmid

#239275

Purpose

Expresses Kif5a*-PYL1 for the anterograde transport of RNA along microtubules via CRISPR-TO in primary mouse cortical neurons

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

Kif5a(aa1-559)-BFP-PYL1 (Kif5a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBI121::chitGFP5:AQ

Plasmid

#240450

Purpose

Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. For details, see Comments.

Depositor

Christoph Plieth

Article

Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

Insert

Fusion of Chitinase signal, smGFP5, and Aequorin

Use

Tags

Expression

Bacterial and Plant

Mutation

GFP5 for expression plants (PMID 9122158); Solubl…

Promoter

Cauliflower mosaic virus 35S promoter (GenBank AJ…

Available since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hIRF5-V5(4D)-LPETG

Plasmid

#237225

Purpose

Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Interferon regulatory factor 5 (IRF5 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

S451D, S453D, S456D, S462D

Promoter

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-hGfi1

Plasmid

#121200

Purpose

inducible expression of hGfi1 in colorectal cancer cells

Depositor

Noah Shroyer

Article

Chen et al Mol Cancer Res. 2019 Mar;17(3):697-708.

Insert

Growth factor independent 1 transcriptional (GFI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P2L

Promoter

Available since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 StoG

Plasmid

#226610

Purpose

All serines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 StoG (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All serines to glycine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 TtoS

Plasmid

#226611

Purpose

All threonines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoS (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All threonine to serine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoA

Plasmid

#226612

Purpose

All glutamines in the LC domain to alanine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoA (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to alanine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 TtoV

Plasmid

#226613

Purpose

All threonines in the LC domain to valine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoV (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All threonine to valine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoG

Plasmid

#226614

Purpose

All glutamines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoG (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to glycine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoN

Plasmid

#226615

Purpose

All glutamines in the LC domain to asparagine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoN (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to asparagine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoS

Plasmid

#226616

Purpose

All glutamines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoS (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to serine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC noYQ_noYT

Plasmid

#226617

Purpose

T7S / Q160S double mutation

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC noYQ_noYT (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

T7S / Q160S double mutation in LC domain to remov…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 wild type

Plasmid

#226596

Purpose

Wild type sequence of FUS LC-RGG1 domains

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 WT (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

wild type LC-RGG1

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_2b)-PGKpuro2ABFP-W

Plasmid

#208415

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_1k)-PGKpuro2ABFP-W

Plasmid

#208414

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)

Plasmid

#238189

Purpose

Lentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

PLAGL1 sgRNA CRISPRa (PLAGL1 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA816 - pBA904 Puro-T2A-GFP ZNF296 g6 CRISPRa guide (pRCA360 backbone) 694

Plasmid

#238179

Purpose

Lentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

ZNF296 sgRNA CRISPRa (ZNF296 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA815 - pBA904 Puro-T2A-GFP ZNF296 g5 CRISPRa guide (pRCA360 backbone) 695

Plasmid

#238178

Purpose

Lentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

ZNF296 sgRNA CRISPRa (ZNF296 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-CKAR2

Plasmid

#236100

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

LAMP1-ExRai-CKAR2 (LAMP1 Human, Synthetic)

Use

Tags

Full-length LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(R78L)

Plasmid

#225517

Purpose

Lentiviral TMEM230(R78L) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

R78L

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(X121PG)

Plasmid

#225518

Purpose

Lentiviral TMEM230(X121PG) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

X121PG

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(X121W)

Plasmid

#225519

Purpose

Lentiviral TMEM230(X121W) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

X121W

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(Y29C)

Plasmid

#225520

Purpose

Lentiviral TMEM230(Y29C) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Y29C

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE

Plasmid

#228766

Purpose

insert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27K

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

p27 kinase inhibitor (Cdkn1b Mouse)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203219

Purpose

Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-QNZF-mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

Plasmid

#231350

Purpose

Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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