Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,769 results for: 109

Showing: 5581 - 5600 of 13769 results

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Desmoplakin II donor only control (FL-based tension sensor)

Plasmid

#118723

Purpose

The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry(Y72L)

Expression

Mammalian

Mutation

inserted FL-based tension sensor module after aa1…

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.P367R

Plasmid

#82851

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

P367R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.W450L

Plasmid

#82830

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

W450L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTW334e2b

Plasmid

#246587

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Mutation

AtBSD2W108A/L109E

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag

Plasmid

#238572

Purpose

Express NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF(RBD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag

Plasmid

#238576

Purpose

Express NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF(RBD-CRD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12D)_BRAF-NanoLuc

Plasmid

#238563

Purpose

Express HaloTag-KRAS(G12D)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12V)_BRAF-NanoLuc

Plasmid

#238565

Purpose

Express HaloTag-KRAS(G12V)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12C)_BRAF-NanoLuc

Plasmid

#238566

Purpose

Express HaloTag-KRAS(G12C)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12C

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-CAG-ARID1A-deltaIDR2-FLAG

Plasmid

#242215

Purpose

Expression of human ARID1A ∆IDR2 (deletion of a.a. 1170-1610) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted IDR2 from ARID1A (deletion of a.a. 1170-1…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta1-3-HA

Plasmid

#242219

Purpose

Expression of human β-catenin ∆arm 1-3 (deletion of a.a. 134-266) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 1-3 from β-catenin (deletion of a.a. …

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-Armadillo-HA

Plasmid

#242224

Purpose

Expression of human β-catenin Armadillo repeats (deletion of a.a. 2-133 and a.a. 665-781) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted N terminal and C terminal IDRs from β-cat…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-deltaC-HA

Plasmid

#242223

Purpose

Expression of human β-catenin ∆C terminal IDR (deletion of a.a. 665-781) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted C terminal IDR from β-catenin (deletion o…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ARID1A-deltaIDR1ARID-FLAG

Plasmid

#242211

Purpose

Expression of human ARID1A ∆IDR1 and ∆ARID (deletion of a.a. 2-1169) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted IDR1 and ARID from ARID1A (deletion of a.…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta4-6-HA

Plasmid

#242220

Purpose

Expression of human β-catenin ∆arm 4-6 (deletion of a.a. 268-394) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 4-6 from β-catenin (deletion of a.a. …

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta10-12-HA

Plasmid

#242222

Purpose

Expression of human β-catenin ∆arm 10-12 (deletion of a.a. 522-664) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 10-12 from β-catenin (deletion of a.a…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129)

Plasmid

#239329

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3B

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 targting UPF3A and 7SK-driven sgRNA2 targeting UPF3B (UPF3B Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-GST-Armadillo

Plasmid

#242225

Purpose

Expression of GST-tagged human β-catenin Armadillo repeats without IDRs (deletion of a.a. 2-133 and 672-781) in E. coli

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Deleted N terminal and C terminal IDRs from β-cat…

Promoter

tac

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-c4X-MBP-ARID1A-IDR2

Plasmid

#242216

Purpose

Expression of MBP-tagged human ARID1A IDR2 (a.a. 1170-1610) in E. coli

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Tags

MBP

Expression

Bacterial

Mutation

ARID1A IDR2 (a.a. 1170-1610)

Promoter

tac

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-deltaN-HA

Plasmid

#242218

Purpose

Expression of human β-catenin ∆N terminal IDR (deletion of a.a. 2-133) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted N terminal IDR from β-catenin (deletion o…

Promoter

CAG

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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