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pCR3.1-OPNc M5

Plasmid

#106325

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site insertion mutant EEKQNA-->EEK…

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M4

Plasmid

#106324

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site deletion mutant EEKQNA-->EEKNA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M3

Plasmid

#106323

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQNAV-->AAAAAAA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEYmRD3A

Plasmid

#127756

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach,RFP Divergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc10-HUHe

Plasmid

#243745

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc10-HUHe

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_Δpvc10

Plasmid

#198448

Purpose

Produces PVCs containing truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_Δpvc10

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 Human WIP

Plasmid

#29573

Depositor

Anna Huttenlocher

Article

Cortesio et al Mol Biol Cell. 2010 Jan 1. 21(1):186-97.

Insert

WASP-interacting protein (WIPF1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Tags

His6-MBP

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNa-delta S

Plasmid

#107983

Purpose

bacterial expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNa 1080bp-17aa signal (SPP1 Human)

Tags

GST

Expression

Bacterial

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNb-delta S

Plasmid

#107984

Purpose

bacterial expression of OPN splice variant b

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNb 1038bp-17aa signal (SPP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNc-delta S

Plasmid

#107985

Purpose

bacterial expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNc 999bp-16aa signal (SPP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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γA3/α1-GFP

Plasmid

#33112

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplaspic domain has been replaced with the cyt…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I Y248&249F

Plasmid

#22189

Depositor

Ananda Roy

Article

Novina et al J Biol Chem. 1998 Dec 11. 273(50):33443-

Insert

TFII-I (GTF2I Human)

Tags

GST

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines.

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf_pvc13-Truncated_pvc10-Cas9

Plasmid

#243734

Purpose

PVC structural operon - untargeted (truncated) tail fiber (Pvc13) - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Truncated_pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10

Plasmid

#243735

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on NTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Cas9-Pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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