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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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EphA2-MT

Plasmid

#108851

Purpose

Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Publication

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EPHA2 (EPHA2 Human)

Tags

Labeled with mTurquoise on the C-Terminus via a 1…

Expression

Mammalian

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti4/TO-hEPO

Plasmid

#50437

Purpose

express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system

Depositor

Juan Melero-Martin

Publication

Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

Insert

EPO (EPO Human)

Use

Lentiviral; Tetracycline-regulatable (t-rex)

Expression

Mammalian

Promoter

CMV/TO

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HA-FRMD6

Plasmid

#32838

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FRMD6 (FRMD6 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRK5-myc-PAT1

Plasmid

#88856

Purpose

myc-TAG overexpression

Depositor

David Sabatini

Publication

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

SLC36A1 (SLC36A1 Human)

Tags

myc

Expression

Mammalian

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTC147

Plasmid

#70013

Purpose

T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.

Depositor

Daniel Voytas

Publication

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

35S:ANT1

Expression

Plant

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#48100

Purpose

Cumate-inducible expression of ECFP-tagged fusion protein

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and ECFP

Expression

Bacterial

Promoter

PQ5

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Publication

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HN

Plasmid

#104171

Purpose

Cas9-WT mammalian expression

Depositor

Jin-Soo Kim

Publication

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-WT

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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NavBp

Plasmid

#100014

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Publication

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NavBp

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pNeoXTR f2

Plasmid

#69159

Purpose

mouse targeting vector

Depositor

David Feldser

Publication

Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

Inserts

DTA
NeoR
GFP
AmpR

Use

Mouse Targeting

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3xFNLDD/pMXs-puro

Plasmid

#49536

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Publication

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

LTR

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP

Use

Lentiviral

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only