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Showing: 5621 - 5640 of 51519 results

  6. pL0_AC-27877

    Plasmid
    #202110
    Purpose
    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    27877 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available Since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202110-plasmid-map-sequence-id-405522

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  7. pL0_CE-OriT

    Plasmid
    #202150
    Purpose
    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Origin of transfer
    Use
    Synthetic Biology
    Mutation
    OriT sequence mutated to remove sapI sites domest…
    Available Since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202150-plasmid-map-sequence-id-405613

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  8. pL0_AC-Fld

    Plasmid
    #202125
    Purpose
    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Fld promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available Since
    Aug. 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202125-plasmid-map-sequence-id-405541

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  9. pL0_AC-54730

    Plasmid
    #202109
    Purpose
    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    54730 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202109-plasmid-map-sequence-id-405433

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  10. pL0_AC-51183

    Plasmid
    #202108
    Purpose
    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    51183 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202108-plasmid-map-sequence-id-405432

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  11. pL0_AC-GEF

    Plasmid
    #202107
    Purpose
    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GEF promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI site for…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202107-plasmid-map-sequence-id-405430

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  12. pL0_AC-47740

    Plasmid
    #202106
    Purpose
    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    47740 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202106-plasmid-map-sequence-id-405429

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  13. pL0_AC-BCA

    Plasmid
    #202105
    Purpose
    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    BCA promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202105-plasmid-map-sequence-id-405428

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  14. pL0_AC-epyC

    Plasmid
    #202104
    Purpose
    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI sites fo…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202104-plasmid-map-sequence-id-405424

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  16. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PR promoter and PLTetO-1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

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  17. pSLiP-G1G2

    Plasmid
    #188965
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188965-plasmid-map-sequence-id-374348

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  19. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

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  20. pSC51

    Plasmid
    #104825
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.12g075700, Glyma.11g145900
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    104825-plasmid-map-sequence-id-203148

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Showing: 5621 - 5640 of 51519 results