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MIGS2.2 (FF573)

Plasmid

#35248

Purpose

Expression of miR173 precursor under UBQ10 promoter; includes miR173 target site after 35S promoter (Kanamycin selection marker)

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Promoter

CaMV 35S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCYP29

Plasmid

#212862

Purpose

Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP58

Plasmid

#212891

Purpose

Expresses the Streptomyces griseolus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP24

Plasmid

#212857

Purpose

Expresses the Streptomyces cyslabdanicus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP63

Plasmid

#212896

Purpose

Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP33

Plasmid

#212866

Purpose

Expresses the Streptomyces platensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP25

Plasmid

#212858

Purpose

Expresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm1

Plasmid

#99355

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm1

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Article

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Promoter

Acetamidase operone

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
pKmK.M1

Plasmid

#125058

Purpose

Storage plasmid for selection marker

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

ScTRP1 marker

Use

Synthetic Biology

Expression

Yeast

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pYS1

Plasmid

#117488

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Article

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

Che9c 60–61, sacB, aph

Use

Deletion generation

Promoter

Acetamide

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUMV19

Plasmid

#67161

Purpose

inducible operation of the mevalonate pathway in E. coli

Depositor

Tomohisa Kuzuyama

Article

Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

Insert

the mevalonate pathway genes

Expression

Bacterial

Promoter

lacZ

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P41nmt1-3FLAG

Plasmid

#19340

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

his3 (his3 Fission Yeast)

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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NK802

Plasmid

#166056

Purpose

Puromycin resistance and mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

Pac puromycin resistance gene

Use

S. rosetta expression

Tags

mCherry

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P81nmt1-3FLAG

Plasmid

#19341

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

his3 (his3 Fission Yeast)

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pTC147

Plasmid

#70013

Purpose

T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

35S:ANT1

Expression

Plant

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pKM343

Plasmid

#71487

Purpose

Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-cat-loxP

Use

Source of loxp-hyg-cat- loxp for pcr amplificatio…

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-Purg1-3FLAG

Plasmid

#19355

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

urg1 promoter

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pR5-28

Plasmid

#22289

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Expression

Yeast

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
pMKInt

Plasmid

#18952

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Insect

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only