167,318 results

1. pcDNA3-FLAG-ATBF1

   Plasmid

   #40927

   Depositor

   Jin-Tang Dong

   Article

   Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

   Insert

   ATBF1 (ZFHX3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   CMV

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-EF1a MICA*009-IRES-ZsGreen

   Plasmid

   #114007

   Purpose

   Induces expression of human MICA allele 009 cDNA

   Depositor

   Kai Wucherpfennig

   Article

   Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

   Insert

   MICA*009 (MICA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM496 (deletions) Zeo

   Plasmid

   #109301

   Purpose

   ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   Bxb1 attB site
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Lamin-A

   Plasmid

   #206027

   Purpose

   Expresses GFP-tagged Lamin A in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Lamin-A (LMNA Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-mRuby2

   Plasmid

   #99114

   Purpose

   An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mRuby2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Expression

   Mammalian

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF1761

   Plasmid

   #142843

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF24 (ZNF24 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV rtTA3 Hygro (w785-1)

   Plasmid

   #26730

   Purpose

   3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor A3 mutant
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG5 Large T

   Plasmid

   #9053

   Depositor

   William Hahn

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME phluorin2-gpi

    Plasmid

    #73793

    Purpose

    ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2-gpi
    

    Use

    Middle entry clone

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE8e(TadA-8e V106W)

    Plasmid

    #138495

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_CreNLS_PP7

    Plasmid

    #232439

    Purpose

    expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cre–MYC-NLS
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid

    #193298

    Purpose

    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-pegRNA-puro backbone

    Plasmid

    #214085

    Purpose

    Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TRE-dCas9-KRAB-MeCP2

    Plasmid

    #122267

    Purpose

    SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Tags

    dCas9-KRAB-MeCP2

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLv-HSA-uDys/eGFP

    Plasmid

    #26810

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    dystrophin (DMD Human)
    

    Use

    Lentiviral; Lentiviral plasmid backbone from l. n…

    Tags

    eGFP

    Mutation

    deleted R4-R23 deleted exons 71-78

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Article

    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Promoter

    EF1alpha and mU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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