161,697 results

1. pHR_AXP_hrGFP

   Plasmid

   #84613

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.3G

   Plasmid

   #14748

   Depositor

   Christophe Benoist, Diane Mathis

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

   Plasmid

   #86157

   Purpose

   FLS2-GFP expresssion

   Depositor

   Silke Robatzek

   Article

   Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

   Insert

   FLS2 (FLS2 Mustard Weed)
   

   Use

   Tags

   3xmyc and GFP

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB-rDA3h

   Plasmid

   #208703

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE Dlg4-GFP KI

   Plasmid

   #131477

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.0-TERT WT

   Plasmid

   #84924

   Purpose

   luciferase reporter for TERT promoter

   Depositor

   Joseph Costello

   Article

   Bell et al Science. 2015 May 29;348(6238):1036-9. d

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   TERT

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6-ARID1A

   Plasmid

   #39311

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-mCherry

   Plasmid

   #41583

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

   Plasmid

   #176285

   Purpose

   Cre-dependent AAV virus

   Depositor

   The Allen Institute for Brain Science, Shenqin Yao

   Article

   Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

   Insert

   TVA66T-dTomato-rabies Glycoprotein
   

   Use

   Tags

   dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pRPS5a

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HDAC6 Flag

    Plasmid

    #13823

    Purpose

    Mammalian expression of human histone deacetylase 6 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Glycogene CRISPR Library

    Pooled Library

    #140961

    Purpose

    Targets human genes involved in cellular glycosylation.

    Depositor

    Sriram Neelamegham

    Article

    Zhu et al Glycobiology. 2020 Aug 8. pii: 5889976.

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pLenti_CMV: Flag-Akaluc_SV40:PuroR

    Plasmid

    #183048

    Purpose

    Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    AkaLuc
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1_blasti

    Plasmid

    #194067

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AID* Kit

    Plasmid Kit

    #1000000120

    Purpose

    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

    Depositor

    Helle Ulrich

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1-IRES eGFP

    Plasmid

    #128059

    Purpose

    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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