We narrowed to 15,991 results for: LEA

1. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CKAR2

   Plasmid

   #123348

   Purpose

   Improved FRET-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   CKAR2
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK351

   Plasmid

   #129696

   Purpose

   For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

   Depositor

   John Heap

   Article

   Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

   Inserts

   PrhaBAD
   yfp
   rhaS
   Terminator ECK120034435
   

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDarmo.CMVT_v1

   Plasmid

   #133072

   Purpose

   Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

   Depositor

   Kacper Rogala, David Sabatini

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   3C-Flag-TwinStrep and TwinStrep-Flag-3C

   Expression

   Mammalian

   Promoter

   CMVT (Enhanced CMV)

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-STIM1

   Plasmid

   #228952

   Purpose

   Retroviral expression of mCherry-STIM1

   Depositor

   David Pellman

   Article

   Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GfaABC1D-GRAB_Ado1.0med

   Plasmid

   #153289

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Promoter

   GfaABC1D

   Available Since

   Aug. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR_L1-YFP-miniTurbo-L2

   Plasmid

   #127352

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-SUMO-hDDI2_pET11a

   Plasmid

   #169120

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKII-GRAB_g5-HT3.0

   Plasmid

   #208723

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Promoter

   CaMKII

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid

    #127239

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA1 Flag ATXN1[2Q]

    Plasmid

    #33235

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    CMV

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-CUL1 K720A

    Plasmid

    #19939

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 K720A (CUL1 Human)
    

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    CUL1 with a NEDD8 modification mutant

    Available Since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-N3-TYK2

    Plasmid

    #139354

    Purpose

    Expression of TYK2 protein fused with GFP

    Depositor

    Sandra Pellegrini

    Article

    Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAS9PBKS

    Plasmid

    #68371

    Purpose

    CbAkt promoter driven Cas9-2A-GFP (from px458)

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    Cas9
    

    Tags

    2A-EGFP

    Expression

    Mammalian

    Promoter

    CbAkt

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPEP-TEV cNup98 (1-498)

    Plasmid

    #38037

    Depositor

    Roderick Lim

    Article

    Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

    Insert

    Nucleoporin 98 (NUP98 Human)
    

    Tags

    His-tag, 3Cysteine

    Expression

    Bacterial

    Mutation

    amino acids 1-498

    Promoter

    T7

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCherry F8-2

    Plasmid

    #186034

    Purpose

    Express VHH F8-2 as a mCherry fusion in mammalian cells

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-YOD1

    Plasmid

    #85664

    Purpose

    Expression of human YOD1 with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    YOD1 (YOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. HypaCas9

    Plasmid

    #138557

    Purpose

    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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