We narrowed to 9,180 results for: mel

1. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

   Plasmid

   #193752

   Purpose

   human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL1RN gRNA3

   Plasmid

   #113132

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. IL1RN gRNA4

   Plasmid

   #113133

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. IL1RN gRNA2

   Plasmid

   #113131

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLKO-mcherry-luc-puro-TRPM1 cDNA

   Plasmid

   #29782

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   TRPM1 (TRPM1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRedCmOcr

   Plasmid

   #176226

   Purpose

   A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PrhaB and PL

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CABE-T1.2

   Plasmid

   #193258

   Purpose

   Expression of CABE-T1.2 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T1.2
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-Cortactin

   Plasmid

   #74476

   Purpose

   This plasmid was constructed to study cortactin interactions

   Depositor

   J Thomas Parsons

   Article

   Weed et al J Cell Biol. 2000 Oct 2;151(1):29-40.

   Insert

   Cortactin (Cttn Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRN3P-HA-Suv4-20h1

   Plasmid

   #86689

   Purpose

   For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
   

   Tags

   HA-tag

   Promoter

   T3

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. RDX-mCherry

    Plasmid

    #214476

    Purpose

    Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    RDX (RDX Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Sequence matches cDNA clone MGC:48283 IMAGE:52844…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3015b

    Plasmid

    #209317

    Purpose

    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

    Depositor

    Thomas Hooven

    Article

    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

    Insert

    Group B Streptococcus-compatible single guide RNA
    

    Expression

    Bacterial

    Promoter

    Xyl/tet promoter that is constitutively active

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CABE-T3.1

    Plasmid

    #193267

    Purpose

    Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.1
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75154

    Purpose

    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabePuro-ApoECDS

    Plasmid

    #42949

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    Apolipoprotein E (APOE Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

16. psiCheck-ApoECDS

    Plasmid

    #41848

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    ApoE CDS (APOE Human)
    

    Use

    Luciferase

    Tags

    renilla luciferase

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-TWIN-STREP-FLAG-NPRL2

    Plasmid

    #164985

    Purpose

    NPRL2 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    NPRL2 (NPRL2 Human)
    

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmiRFP703-Clathrin

    Plasmid

    #79997

    Purpose

    Clathrin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Clathrin (CLTB Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYO1273

    Plasmid

    #235746

    Purpose

    Expression of FL human EGFP-ATG14L in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Insert

    ATG14L (ATG14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAWPSG-Ptet-nCas9-BE

    Plasmid

    #195739

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptet(Tetracycline inducible protein)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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