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pCAMBgfp

Plasmid

#206130

Purpose

This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

Depositor

Anne Osbourn

Article

Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

Insert

hygromycin resistance gene and the Promoter of ToxA+SGFP

Use

Binary vector for expression of sgfp in filamento…

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein

Plasmid

#73519

Purpose

Encodes mouse ninein tagged with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrC

Plasmid

#174615

Purpose

Bacterial expression of mtrC from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

MtrC

Use

Synthetic Biology; Shuttle vector in e. coli and …

Expression

Bacterial

Promoter

pLacI

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5R MCS+

Plasmid

#211354

Purpose

empty vector control

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA221TadA*-T7RNAP

Plasmid

#167980

Purpose

Expresses fusion protein TadA*-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TadA*-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmec-4::Chrimson::SL2::mCherry::unc-54

Plasmid

#107745

Purpose

Express Chrimson and mCherry in mechanosensory neurons.

Depositor

Andrew Leifer

Article

Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

Inserts

Chrimson
mCherry

Expression

Insect and Worm

Promoter

mec-4

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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Lbpro (29-195)

Plasmid

#110759

Purpose

Bacterial expression of Lbpro

Depositor

Tim Skern

Article

Guarne et al EMBO J. 1998 Dec 15;17(24):7469-79. doi:

Insert

Lb pro

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-Tandem-mCherry-EGFP

Plasmid

#123216

Purpose

N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pACT2-Kre33

Plasmid

#99990

Purpose

Express yeast Kre33 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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LT1_33_pTEFmut8-mCherry

Plasmid

#177706

Purpose

Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEFmut8-mCherry

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

Plasmid

#225924

Purpose

Expressing LGC-47 in AVA neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

rig-3

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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C413-E38: attB4-T7p>-attB1r

Plasmid

#162929

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204778

Purpose

Bacterial expression of Sars Cov 2 MPRO M165I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M165I

Available Since

Dec. 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRK1037

Plasmid

#8833

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease, catalytic domain

Expression

Bacterial

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-mRuby3

Plasmid

#179730

Purpose

Mammalian expression of mRuby3 from the EF1 promoter

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

mRuby3

Expression

Mammalian

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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