We narrowed to 15,991 results for: LEA

1. pcDNA3-Lyso-mCherry-Rheb(C181S)

   Plasmid

   #192438

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   Lyso-mCherry-Rheb(C181S) (RHEB Human)
   

   Tags

   Full-length LAMP1 and mCherry

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA6A-EGFR ICD (645-1186)

   Plasmid

   #42667

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR ICD (645-1186) (EGFR Human)
   

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pDisplay-GRAB_g5-HT2h

   Plasmid

   #208709

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2h
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TUBG1

   Plasmid

   #87954

   Purpose

   expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

   Depositor

   Maria Alvarado-Kristensson

   Article

   Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   whole protein, The C-terminal is a sh-gamma-tubul…

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

   Plasmid

   #122083

   Purpose

   Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-LaminB1
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of LaminB1

   Expression

   Bacterial and Mammalian

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Akt-STOPS-NES

   Plasmid

   #175008

   Purpose

   Cytosol-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

   Plasmid

   #169921

   Purpose

   DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   IRES-Puro-pEF1a-EGFP
   

   Use

   CRISPR

   Promoter

   EF1a

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CS2-Myc-hASUN

   Plasmid

   #47955

   Purpose

   Expression of tagged-human ASUN

   Depositor

   Laura Lee

   Article

   Jodoin et al Mol Biol Cell. 2013 Jul 31.

   Insert

   Asunder (INTS13 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   Sp6

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

9. SpCas9-NG-CGBE1

   Plasmid

   #198537

   Purpose

   Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSOMA-NLS

    Plasmid

    #118937

    Purpose

    Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-WT (AF41)

    Plasmid

    #21367

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205320

    Purpose

    All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAba30 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSOMA-NES

    Plasmid

    #118939

    Purpose

    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPPEDs

    Plasmid

    #162469

    Purpose

    protoplast test vector of pPPED

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Prime editing destination vector for transient ex…

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV2-miniSOG-VAMP2-T2A-mCherry

    Plasmid

    #50970

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-T2A-mCherry (Vamp2 Mouse, Arabdopsis thaliana)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. myristoylated ct-GRK2

    Plasmid

    #178734

    Purpose

    N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

    Depositor

    Ege Kavalali

    Article

    Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

    Insert

    GRK2 (GRK2 Bovine)
    

    Use

    Lentiviral

    Tags

    Src 1-15

    Mutation

    only amino acids 548-671

    Promoter

    Ubiquitin

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO-VMAT2-WPRE-pA

    Plasmid

    #39339

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV; R

    Mutation

    Myc tag inserted in the first lumenal loop betwee…

    Available Since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1a-Neuromodulin-IRES-Puromycin

    Plasmid

    #134666

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Neuromodulin (GAP43 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-G2-ATP8-V5 (puromycin)

    Plasmid

    #86852

    Purpose

    mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Tags

    ATP5G2 (G2) mitochondrial targeting signal and V5

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKII-STChRger2-TS-EYFP

    Plasmid

    #129393

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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