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pGEX-4T1-Claspin-BPII

Plasmid

#31632

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 492-622 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-myc-CD4-Sema4D

Plasmid

#51603

Purpose

expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4-Sema4D

Tags

myc

Expression

Mammalian

Mutation

Sema4D amino acids 1-754 have been replaced with …

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pMSP1D1T1

Plasmid

#97327

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1T1

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of T residue after T25

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMSP1D1T2

Plasmid

#97328

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1T2

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of two T residues after T25

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pconst #3-RFP

Plasmid

#81077

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23101

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8.3

Plasmid

#121959

Purpose

Mammalian expression, Transcription regulation, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, MS2 hairpin inserted

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV T274V S392A KSR1 IRES GFP

Plasmid

#25979

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine and serine 392 to…

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

Plasmid

#46838

Purpose

Express cGFP transcript ending in the minimal functional MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST_EWSR1_G2A_R650H

Plasmid

#26383

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

R650H

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pENTR4_EWSR1_G2A_R650H

Plasmid

#26372

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Mutation

R650H

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
MSCV T274V KSR1 IRES GFP

Plasmid

#25977

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
MSCV S392A KSR1 IRES GFP

Plasmid

#25978

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pTGC-U6.2

Plasmid

#112809

Purpose

PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:2-gRNAcore

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pOPTXGARE

Plasmid

#68542

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with GARE (AT1G33440 Mustard Weed)

Tags

luciferase

Expression

Bacterial and Plant

Mutation

5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

Promoter

OPTX promoter with GARE

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMGC

Plasmid

#112812

Purpose

PCR template vector for amplifying gRNA(F+E)-OstRNA fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

gRNAcore(F+E)-tRNA

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 308-579

Plasmid

#11632

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal common domain of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pRKVSV FRNK

Plasmid

#50534

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2186-3268)

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pBS/U6/hTID1

Plasmid

#13745

Depositor

Karl Munger

Article

Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

Insert

hTid1 siRNA (DNAJA3 Human)

Use

RNAi

Mutation

Inverted repeat of 21 bp with 6 nuc. spacer.

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only