We narrowed to 13,634 results for: mpl

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pcDNA3-HA-REDD1 S137D (#628)

Plasmid

#99944

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S137D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S103W (#623)

Plasmid

#99941

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S103W

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pTC130

Plasmid

#91178

Purpose

BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALENs targeting tomato ANT1

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 313

Plasmid

#98586

Purpose

pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

csrB

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 314

Plasmid

#98584

Purpose

pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Inserts

CsrA
csrB

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT1

Plasmid

#79499

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT1 (WNT1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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GST-peripherin-CTS

Plasmid

#86070

Purpose

A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Peripherin (prph2.S Frog)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 317-336 of peripherin

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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GST-prRDH-CTS

Plasmid

#86069

Purpose

A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Retinol dehydrogenase (RDH8 Bovine)

Tags

GST

Expression

Bacterial

Mutation

the insert is 297-312 of RDH8 (Bos taurus)

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSI HA Nup53xl

Plasmid

#86000

Purpose

mammalian expression of HA tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

HA

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pVH009

Plasmid

#80399

Purpose

Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

3xFLAG-Venus YFP-AAV
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal LZx domain and N-terminal 3xFLAG tag, …

Expression

Bacterial

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE NLS

Plasmid

#82465

Purpose

Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Capping Enzyme (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Mutation

deleted amino acids Nuclear Localization Signal

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE

Plasmid

#82463

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt HIV, Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(211-597)

Plasmid

#82462

Purpose

Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE(211-597) (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBW301ParsR-Amp30T

Plasmid

#78653

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-Zeo-NKX2-8

Plasmid

#75090

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-8 (NKX2-8 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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Zebrafish lim5 (lhx5)

Plasmid

#74141

Purpose

Basic cDNA clone for zebrafish lim5 (lhx5). Cloned in BS KS+.

Depositor

Igor Dawid

Article

Toyama et al Dev Biol. 1995 Aug;170(2):583-93.

Insert

lhx5 (lhx5 Zebrafish)

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 G108C/P186C

Plasmid

#66174

Purpose

Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C, P186C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

EE

Expression

Mammalian

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only