We narrowed to 9,497 results for: pho;

1. pLV-REPORT(EF1a no GFP) mnuctdTomato

   Plasmid

   #172342

   Purpose

   mnuctdTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB330

   Plasmid

   #60147

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   NLuc
   

   Tags

   Nuclear localization signal (NLS)

   Expression

   Yeast

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-RARa-VP16

   Plasmid

   #185559

   Purpose

   Ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

   Depositor

   Connie Cepko

   Article

   Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

   Insert

   RARa-VP16 (Rara Mouse)
   

   Tags

   VP16

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 274_pSKB3-Venus-LUMP

   Plasmid

   #65976

   Purpose

   Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Insert

   LUMP
   

   Tags

   Venus and thrombin site (11-residue linker that h…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCerulean3-Rab4a-7

   Plasmid

   #55443

   Purpose

   Localization: Ras GTPase, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Rab4a (RAB4A Human)
   

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a - CPX

   Plasmid

   #89620

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Cytosolic Tryparedoxin peroxidase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK 3XFLAG-MKK4-EE-NLS hygro

   Plasmid

   #87779

   Purpose

   Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

8. mCerulean3-Zyxin-6

   Plasmid

   #55454

   Purpose

   Localization: Focal Adhesions, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Zyxin (ZYX Human)
   

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pmirGLO-Smad5-1719

   Plasmid

   #49382

   Purpose

   Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

   Depositor

   Martine Roussel

   Article

   Murphy et al Cancer Res. 2013 Oct 21.

   Insert

   Smad5 (Smad5 Mouse, Human)
   

   Use

   Luciferase

   Promoter

   Human phosphoglycerate kinase (PGK) promoter

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-IP3KC

    Plasmid

    #32527

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

    Insert

    IP3KC (ITPKC Human)
    

    Tags

    6xHIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

12. mOrange2-Endo-14

    Plasmid

    #57955

    Purpose

    Localization: Endosomes, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Endo (RHOB Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pZyxin-mMaple2

    Plasmid

    #101150

    Purpose

    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    human codon optimized mMaple2 fused to zyxin
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG3H-U6EC1

    Plasmid

    #134758

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA-U6, zCas9-EC1

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 823 pGEX2T PTEN G129R

    Plasmid

    #10749

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G129R

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pLX302-PTP-SL-V5 puro

    Plasmid

    #87774

    Purpose

    Expresses V5 tagged human PTP-SL

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    PTP-SL (PTPRR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a - SodB

    Plasmid

    #89613

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase B
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG

    Plasmid

    #29583

    Depositor

    Anna Huttenlocher

    Article

    Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

    Insert

    phosphatidylinositol phosphate kinase type I gamma
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Feb. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBL119-2

    Plasmid

    #104336

    Purpose

    Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    Thiosulfate response regulator
    

    Expression

    Bacterial

    Promoter

    PLTetO1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFIN-EF1a-CHER-WPRE

    Plasmid

    #44353

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    EF1a-CHER
    

    Use

    Lentiviral

    Promoter

    human elongation factor -1a (pos 2526-3969)

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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