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pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Promoter

CMV-tet-ON

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)

Plasmid

#191630

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadS

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)

Plasmid

#191645

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)

Plasmid

#191646

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRD424

Plasmid

#178202

Purpose

Expression of Rok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + rok

Expression

Bacterial

Promoter

hns promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRD410

Plasmid

#178200

Purpose

Expression of sRok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + srok

Expression

Bacterial

Promoter

hns promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

Plasmid

#191481

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR ΔSL1

Use

Lentiviral and Luciferase

Mutation

Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184692

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184693

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184694

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184701

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184702

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB116.2

Plasmid

#181946

Purpose

aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

TorR-mNG; mCherry; tetR (AX04_RS23485, torR torR - E. coli, Synthetic)

Tags

mNeonGreen

Expression

Bacterial

Promoter

TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB120.2

Plasmid

#181947

Purpose

aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNG-TorR; mCherry; tetR (torR, AX04_RS23485 torR - E. coli, Synthetic)

Tags

mNeonGreen

Expression

Bacterial

Promoter

mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Tags

mNeonGreen

Expression

Bacterial

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.3.hSyn.H2B.RFP

Plasmid

#170370

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Ovol1

Plasmid

#172871

Purpose

Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Ovol1 (Ovol1 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.13.hSyn.H2B.RFP

Plasmid

#170372

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.4.EFS-NS.H2B-RFP

Plasmid

#170365

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.3.EFS-NS.H2B-RFP

Plasmid

#170364

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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