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pEYFP-N1-HsSmg6_817-1238_I

Plasmid

#146560

Purpose

Mammalian Expression of HsSmg6_817-1238-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_817-1238-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6_1239-1419_I

Plasmid

#146561

Purpose

Mammalian Expression of HsSmg6_1239-1419-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1239-1419-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6-del36-576-siRNAres_I

Plasmid

#146562

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg6_577-816-siRNAres-V5-SBP-MBP_I

Plasmid

#146570

Purpose

Mammalian Expression of HsSmg6_577-816-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_577-816-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg6_817-1238-V5-SBP-MBP_I

Plasmid

#146571

Purpose

Mammalian Expression of HsSmg6_817-1238

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_817-1238 (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg6_1239-1419-V5-SBP-MBP_I

Plasmid

#146572

Purpose

Mammalian Expression of HsSmg6_1239-1419

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1239-1419 (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_D17A-V18A

Plasmid

#194184

Purpose

Expresses ZNF354C with mutated KRAB domain (D17A, V18A mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant D17A/V18A

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_E25A-W26A

Plasmid

#194186

Purpose

Expresses ZNF354C with mutated KRAB domain (E25A, W26A mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant E25A/W26A

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEY91

Plasmid

#191084

Purpose

glr-4 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-4 (glr-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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GB2890

Plasmid

#193133

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1dG2b.1

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GB1839

Plasmid

#193103

Purpose

GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP gRNA2

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY89

Plasmid

#191082

Purpose

glc-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glc-3 (glc-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY83

Plasmid

#191078

Purpose

flp-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-8 (flp-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY78

Plasmid

#191073

Purpose

dop-6 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dop-6 (dop-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY65

Plasmid

#191062

Purpose

bas-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

bas-1 (bas-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::HA-RfA

Plasmid

#186404

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEY86

Plasmid

#191079

Purpose

gcy-25 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-25 (gcy-25 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY87

Plasmid

#191080

Purpose

gcy-27 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-27 (gcy-27 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY90

Plasmid

#191083

Purpose

glr-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-2 (glr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY92

Plasmid

#191085

Purpose

glr-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-8 (glr-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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