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LentiCRISPR-B_2xsgRNAs_SKIV2L2 (pAVA3251)

Plasmid

#239323

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting SKIV2L2

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting SKIV2L2 (MTREX Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_ZCRB1 (pAVA3300)

Plasmid

#239325

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNA stargeting ZCRB1

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting ZCRB1 (ZCRB1 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)

Plasmid

#239296

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting C1D (C1D Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)

Plasmid

#239302

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC4 (EXOSC4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC5 (pP18(T)_B12-AVA4068)

Plasmid

#239303

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC5

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC5 (EXOSC5 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3-T181A

Plasmid

#197556

Purpose

Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)

Depositor

Joseph Gordon

Article

Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.

Insert

BCL2 Interacting Protein 3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Threonine 181 changed to alanine

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7V51G-VA

Plasmid

#115183

Purpose

Lentiviral transduction and expression of PARK7V51G into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7V51G (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.V51G

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7C53A-VA

Plasmid

#115184

Purpose

Lentiviral transduction and expression of PARK7C53A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7C53A (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.C53A

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7H126A-VA

Plasmid

#115185

Purpose

Lentiviral transduction and expression of PARK7H126A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7H126A (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.H126A

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7E163K-VA

Plasmid

#115186

Purpose

Lentiviral transduction and expression of PARK7E163K into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7E163K (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.E163K

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeEH258A

Plasmid

#129122

Purpose

expresses H258A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeES157A

Plasmid

#129121

Purpose

expresses S157A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4 WW2 neg ctrl

Plasmid

#112030

Purpose

Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Insert

NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry

Tags

Split mCherry

Expression

Bacterial

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
AA245-428-GFP

Plasmid

#221174

Purpose

Expresses mammalian Arl13b with amino acids 245-428 and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-244

Promoter

CMV promoter

Available Since

May 22, 2026

Availability

Academic Institutions and Nonprofits only

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Corona_Wuhan_RBD

Plasmid

#251256

Purpose

To express the SARS-CoV-2 (Wuhan) receptor binding domain in E. coli

Depositor

Yutaka Kuroda

Article

Brindha et al Int J Mol Sci. 2022 Feb 1;23(3):1703. do

Insert

Corona_Wuhan_RBD (S SARS-CoV-2 Wuhan variant)

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Mutation

C9A

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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His-AA195-428-GFP

Plasmid

#247241

Purpose

A bacterial expression plasmid encoding Arl13b (aa195-428) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

delete amino acids 1-194

Promoter

T7 Promoter

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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His-AA1-245-GFP

Plasmid

#247240

Purpose

A bacterial expression plasmid encoding Arl13b (aa1-245) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

deleted amino acids 246-428

Promoter

t7

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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AA1-245-GFP

Plasmid

#247082

Purpose

Expresses mammalian Arl13b with amino acids 1-245 and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 246-428

Promoter

CMV promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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PAL-13bCTS-GFP

Plasmid

#221592

Purpose

Expresses the palmitoylation sequence, the ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acid 10-346,364-428

Promoter

CMV promoter

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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