We narrowed to 14,359 results for: cas9

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pXSE901B

Plasmid

#91713

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

Depositor

Qi-Jun Chen

Publication

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

Plasmid

#115487

Purpose

CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPN10-gCTG

Plasmid

#114385

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

Depositor

Vincent Dion

Publication

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gCTG

Use

CRISPR

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSPneoHHgRNAH

Plasmid

#63557

Purpose

Expresses gRNA in Leishmania

Depositor

Greg Matlashewski

Publication

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

guide sequence

Use

Leishmania expression

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pDIV270

Plasmid

#226181

Purpose

Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Publication

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJH1

Plasmid

#154204

Purpose

encodes CRISPR-Cas9 system derived from plasmid pCas9

Depositor

Russell Poulter

Publication

Ho et al J Med Microbiol. 2020 Mar;69(3):478-486.

Type

Empty backbone

Use

CRISPR

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEG302 5aa SunTag VP64 nog

Plasmid

#115480

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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Adeno FT

Plasmid

#101824

Purpose

Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3

Depositor

Andrea Ventura

Publication

Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

Insert

U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)

Use

Adenoviral

Tags

FLAG-Cas9

Promoter

U6 and CBh

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJOG252

Plasmid

#80584

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGoldenGreenGate-M (pGGG-M)

Plasmid

#165422

Purpose

A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Publication

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Level 2 golden gate (moclo) du…

Promoter

Contain GG cassette and MCS with LacZ for blue/wh…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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U6>sgRNA(F+E)

Plasmid

#59986

Purpose

Empty vector for sgRNA cloning (F+E modified backbone)

Depositor

Lionel Christiaen

Publication

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Type

Empty backbone

Use

CRISPR

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAGM65879

Plasmid

#153214

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179070

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondria

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179068

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179067

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179066

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-ATG8

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179060

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179052

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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