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We narrowed to 14,582 results for: eng

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Showing: 5761 - 5780 of 14582 results

  2. LARS_pNIC-Bio3

    Plasmid
    #153052
    Purpose
    Expression construct for producing Avi tagged Leucyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    LARS (LARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Not full length protein
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153052-plasmid-map-sequence-id-304468

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  3. TBXTA-c028

    Plasmid
    #139765
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139765-plasmid-map-sequence-id-296580

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  6. HA-IFITM3-F8-TAG-C72A

    Plasmid
    #122477
    Purpose
    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122477-plasmid-map-sequence-id-237728

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  8. pJOG641

    Plasmid
    #105427
    Purpose
    Synthetic biology
    Depositor
    Johannes Stuttmann
    Publication
    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-VP16-GR (transcription factor: inducible expression; dexamethasone)
    Use
    Synthetic Biology
    Mutation
    BsaI/ BpiI restriction sites removed
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105427-plasmid-map-sequence-id-207438

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  9. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Publication
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  16. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  18. pJZC73

    Plasmid
    #62341
    Purpose
    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62341-plasmid-map-sequence-id-102264

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Showing: 5761 - 5780 of 14582 results