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We narrowed to 826 results for: 3xha

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pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

Plasmid

#107327

Purpose

Expresses SpCas9 fused to dNmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107319

Purpose

Expresses SpCas9 fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107318

Purpose

Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107324

Purpose

Expresses SpCas9 fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to NmCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

Plasmid

#107323

Purpose

Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

Plasmid

#69224

Purpose

Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS and Zif268

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107325

Purpose

Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to NmCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107326

Purpose

Expresses SpCas9 (R1333S) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to NmCas9

Promoter

CMV IE93

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

Plasmid

#69232

Purpose

Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130691

Purpose

Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Use

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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