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We narrowed to 4,399 results for: ARA-2

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pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Syntaxin4-myc-myc-His

Plasmid

#12377

Depositor

Thomas Weimbs

Article

Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Insert

Syntaxin4 (STX4 Human)

Use

Tags

2xMyc-His

Expression

Mammalian

Mutation

Cloned in modified PCDNA4 vector (double myc)

Promoter

CMV⁄TO

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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HBT_pcoCASphi_version2

Plasmid

#197932

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

2xNLS-2xFLAG

Expression

Plant

Mutation

Promoter

35SPPDK promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV and cmv

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
p16 nlsGPS2

Plasmid

#228093

Purpose

Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

Depositor

Alexander Jones

Article

Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

Insert

nlsGPS2

Use

Tags

6xHis-T7 XpressTag and cMyc

Expression

Plant

Mutation

Promoter

p16

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCRY2PHR-mCherryN1

Plasmid

#26866

Purpose

Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2PHR (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mamlian codon optimized CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pKERepPar

Plasmid

#211827

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2

Plasmid

#28243

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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B2375_CRY2olig-mEGFP

Plasmid

#215109

Purpose

Expresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CRY2olig-mEGFP (CRY2 Mustard Weed)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

E490G, 1-498

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 S6K2 E388 D3E

Plasmid

#17731

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

Promoter

CMV

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ spike_del19

Plasmid

#155297

Purpose

Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

Depositor

Raffaele De Francesco

Article

Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

Insert

SARS-Cov2 spike_deleted (S SARS-Cov2)

Use

Generation of sars-cov-2-pseudotyped lentiviral p…

Tags

Expression

Mutation

Deletion in C-term 19 aa of spike

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p11-3’_8xN_PAM-site2 (RTW555)

Pooled Library

#160133

Purpose

Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Depositor

Benjamin Kleinstiver, Keith Joung

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
pHM092

Plasmid

#225526

Purpose

Encodes rtTA under AR8 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHM093

Plasmid

#225525

Purpose

Encodes rtTA under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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