We narrowed to 2,779 results for: SAB

1. lentiCRISPR - C16orf80 sgRNA 1

   Plasmid

   #70651

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. lentiCRISPR - C16orf80 sgRNA 2

   Plasmid

   #70650

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. FH95pUP95S73DGW (F3)

   Plasmid

   #74040

   Purpose

   lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

   Depositor

   Robert Malenka, Weifeng Xu, Bernardo Sabatini

   Article

   Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

   Insert

   Psd95
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Mutation

   S73D

   Promoter

   H1

   Availability

   Academic Institutions and Nonprofits only

5. pRK5-FLAG-C7orf59

   Plasmid

   #42332

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   C7orf59 (LAMTOR4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLJC5-LAMP1-RFP-3xHA

   Plasmid

   #102932

   Purpose

   Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   RFP-HA

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLJC5-LAMP1-RFP-2xFlag

   Plasmid

   #102931

   Purpose

   Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   mRFP-2xFlag

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-Cas9-GFP

   Plasmid

   #86145

   Purpose

   Lentiviral vector expressing Cas9-GFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   Cas9-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-TRE-DIO-ChR2-TdTomato

   Plasmid

   #166600

   Purpose

   Encodes Cre-dependent ChR2-TdTomato under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   ChR2-TdTomato
   

   Use

   AAV

   Mutation

   H134R

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcw107

    Plasmid

    #62511

    Purpose

    Gateway Expression Vector

    Depositor

    David Sabatini, John Doench

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. myc-Rictor corrected

    Plasmid

    #11367

    Purpose

    Mammalian expression of myc tagged human RICTOR

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-TRE-DIO-eGFP-2A-TetanusToxin

    Plasmid

    #166603

    Purpose

    Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIS1-Eef25UTR-renilla

    Plasmid

    #38235

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2 5'UTR / Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Eef2 5' UTR fused to renilla ORF

    Promoter

    Endogenous Eef2

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIS1-Eef25UTR-TOPmut-renilla

    Plasmid

    #38236

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    The first nucleotides of the 5' UTR were cha…

    Promoter

    Endogenous Eef2

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs-DDX3X

    Plasmid

    #70648

    Purpose

    Expresses WT DDX3X in mammalian cells under blasticidin selection.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Retroviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

    Plasmid

    #37082

    Purpose

    Cre-dependent expression of hChR2(H134R) for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

    Insert

    hChr2(H134R)-mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    mCherry

    Promoter

    EF1a

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs_FLAG-NMNAT1

    Plasmid

    #133259

    Purpose

    Express FLAG-tagged NMNAT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT1 (NMNAT1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge