We narrowed to 1,368 results for: p65

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pEBG-Pak1 83-149

Plasmid

#12214

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Mammalian

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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EC2_4_dCas9_VPR_sgRNA

Plasmid

#163709

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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CFP-PBD

Plasmid

#11394

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Tags

CFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
YFP-PBD

Plasmid

#11407

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 PBD (PAK1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-MCP-VPR-2AmScarlet

Plasmid

#121964

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64

Plasmid

#101110

Purpose

Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(C)_DHFR-PCP-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B R398,399Q IRES GFP

Plasmid

#133819

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

R398,399Q

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B K326,327E IRES GFP

Plasmid

#133820

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K326,327E

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B D363,365N IRES GFP

Plasmid

#133822

Purpose

Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D363,365N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A D230,232N IRES GFP

Plasmid

#133824

Purpose

Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D230,232N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G F234C

Plasmid

#170647

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G, C277A, F234C

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G V304C

Plasmid

#170648

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G, C277A, V304C

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D365E F234C

Plasmid

#170650

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E, C277A, F234C

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D365E V304C

Plasmid

#170651

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E, C277A, V304C

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) I367T F234C

Plasmid

#170653

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T, C277A, F234C

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) I367T V304C

Plasmid

#170654

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T, C277A, V304C

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1

Plasmid

#213628

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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dSaCas9-VPR

Plasmid

#205251

Purpose

In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSaCas9-VPR

Use

CRISPR

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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dSpCas9-VPR

Plasmid

#205247

Purpose

In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSpCas9-VPR

Use

CRISPR

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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