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pUBC(h)-DEST-mKOk

Plasmid

#224830

Purpose

Empty Gateway DEST vector for expressing C-terminal tagged proteins with mKusabiraOrangekand hygromycin selection in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Type

Empty backbone

Tags

mKusabiraOrangek

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pOSV00157

Plasmid

#222937

Purpose

Kill switch plasmid for DNA sensor construction using Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

TxpA-RatA toxin-antitoxin

Expression

Bacterial

Promoter

Phyperspank

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Nterm_His

Plasmid

#220478

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Nterm_His

Plasmid

#220479

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Nterm_His

Plasmid

#220480

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1377_Nterm_His

Plasmid

#220481

Purpose

heterologous expression of PA1377 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLm_gRNA1

Plasmid

#178752

Purpose

Expression of a gRNA that targets next to PAM library, used for L. mobilis type I-E system

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

a gRNA that targets next to PAM library, used for L. mobilis type I-E system

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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FGFR2 K659M 3xFlag

Plasmid

#110112

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 659 to Methiionine (K659M)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 E565A 3xFlag

Plasmid

#110109

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Glutamic acid 565 to Alanine (E565A)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 K641R 3xFlag

Plasmid

#110111

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 641 to Arginine (K641R)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 N549H 3xFlag

Plasmid

#110106

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Asparagine 549 to Histidine (N549H)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 L617V 3xFlag

Plasmid

#110110

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Leucine 617 to Valine (L617V)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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CMYA5tnT/pDEST26-N-FLAG

Plasmid

#98131

Purpose

Express CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vector

Depositor

Rita Shiang

Insert

CMYA5 (CMYA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

rs10043986 (NM_153610.4:c.12188C>T)

Promoter

CMV

Available Since

Aug. 3, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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phtsResA

Plasmid

#25914

Depositor

Johann Deisenhofer

Article

Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

Insert

His-tagged soluble ResA (resA Bacillus subtilis)

Tags

His

Expression

Bacterial

Mutation

Start at Serine 37 to remove n-terminal transmemb…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pMZ3012

Plasmid

#204400

Purpose

A C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator rhlR (rhlR )
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PRhl0L0L12

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKS17

Plasmid

#24628

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

pdc, adhB, atfA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pTREX GPR68_NB2_2

Plasmid

#127691

Purpose

Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

GPR68_NB2_2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 5HT2A_NB2_1

Plasmid

#127690

Purpose

Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

5HT2A_NB2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWR

Plasmid

#112900

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-MT-mitfa

Plasmid

#81231

Purpose

expression of zebrafish mitfa

Depositor

James Lister, David Raible

Article

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Zebrafish expression

Tags

6 myc tags

Expression

Mammalian

Mutation

contains amino acids 5-412

Promoter

sCMV IE94 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shGL2

Plasmid

#60004

Purpose

Co-expresses control GL2 shRNA and GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Expression

Mammalian

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-SacB-Cas9-T2A-mCherry_(BbsI)

Plasmid

#117070

Purpose

Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

Depositor

Kim Failor

Inserts

Cas9
SacB

Use

CRISPR

Tags

3x FLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ1001

Plasmid

#204398

Purpose

A pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator RpaR (rpaR )
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PRpa0002

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDownstream1Lox

Plasmid

#164574

Purpose

Integration of 1 LoxN site in the genome of P. berghei

Depositor

Olivier Silvie

Type

Empty backbone

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRoCascade_gfp

Plasmid

#178771

Purpose

Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRoDonor

Plasmid

#178773

Purpose

Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0009

Plasmid

#227572

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392 (A357L)_35S

Expression

Plant

Mutation

A357L, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0010

Plasmid

#227573

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392 (H424R)_35S

Expression

Plant

Mutation

H424R, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0018

Plasmid

#227574

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392 (A285V)_35S

Expression

Plant

Mutation

A285V, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0012

Plasmid

#227575

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A393 (A285G)_35S

Expression

Plant

Mutation

A285G, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0014

Plasmid

#227576

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A393 (A357L)_35S

Expression

Plant

Mutation

A357L, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0015

Plasmid

#227577

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A393 (H424R)_35S

Expression

Plant

Mutation

H424R, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0021

Plasmid

#227578

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_ CYP716A393 (A285V)_35S

Expression

Plant

Mutation

A285V, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0029

Plasmid

#227556

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoMAS (I367M)_35S

Expression

Plant

Mutation

I367M, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0030

Plasmid

#227557

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoMAS (E371D)_35S

Expression

Plant

Mutation

E371D, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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