We narrowed to 8,454 results for: gnal

1. CMV-10-3xFLAG-LGP2-MIIa

   Plasmid

   #58684

   Purpose

   Expresses LGP2 with a mutation to motif IIa in mammalian cells

   Depositor

   Curt Horvath

   Article

   Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

   Insert

   LGP2-MIIa (DHX58 Human)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   mutation to Motif IIA, K138E and Y142F

   Promoter

   CMV

   Available Since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

2. FLAG-JAK1

   Plasmid

   #174572

   Purpose

   Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   JAK1 (JAK1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV hSyn INTRON mCherry-Jph3 WPRE

   Plasmid

   #236237

   Purpose

   AAV expression of human Junctophilin3 N-terminally tagged with mCherry

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   Junctophilin 3 (JPH3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV x EF1a_EKAREN4-ires-puro

   Plasmid

   #167828

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV x EF1a_EKAREN5-ires-puro

   Plasmid

   #167821

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. peGFP-N1-Cofilin

   Plasmid

   #186747

   Purpose

   Expresses wildtype GFP-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MED12_pLX307

   Plasmid

   #98350

   Purpose

   Lentiviral expression of MED12

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   MED12 (MED12 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R1392Q

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/TO/GFP-GIGYF2

    Plasmid

    #141189

    Purpose

    Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    GIGYF2 (GIGYF2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmCherry-N1-cofilin

    Plasmid

    #186750

    Purpose

    Expresses wildtype mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. peGFP-N1-Cofilin-S41A

    Plasmid

    #186749

    Purpose

    Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed serine 41 to alanine

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCherry-N1-Cofilin-S41E

    Plasmid

    #186751

    Purpose

    Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed serine 41 to glutamic acid

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. peGFP-N1-Cofilin-S41E

    Plasmid

    #186748

    Purpose

    Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed serine 41 to glutamic acid

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-CMV-Blast-DNFGFR1-HA

    Plasmid

    #130887

    Purpose

    Expresses dominant negative mutant mouse FGFR1

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-mCherry-ARHGEF2

    Plasmid

    #207959

    Purpose

    Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    ARHGEF2 (ARHGEF2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3 Flag MKK7B2Jnk2a2

    Plasmid

    #19727

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Mouse, Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pTR01F/hFGFR2c-V5

    Plasmid

    #236013

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2c protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2c (FGFR2 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV hSyn INTRON HaloTag-Jph3 WPRE

    Plasmid

    #236238

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    HaloTag

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge