We narrowed to 5,840 results for: pCas

1. PX458-sgEIF4EBP1

   Plasmid

   #128097

   Purpose

   CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC763 - pX458 with rat Rosa26 gRNA A

   Plasmid

   #113161

   Purpose

   A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat Rosa26
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-PE SpRY

   Plasmid

   #179082

   Purpose

   NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

   Promoter

   CAG

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

   Plasmid

   #154429

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

   Plasmid

   #198751

   Purpose

   Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - LargeBiT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

   Plasmid

   #198750

   Purpose

   Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - SmallBiT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgTP53(A)

   Plasmid

   #85559

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

8. peSCBE3-NG-HF1-Hypa

   Plasmid

   #218166

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgTP53(B)

   Plasmid

   #85560

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

    Plasmid

    #217805

    Purpose

    Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. peSCBE3-NG-HF1

    Plasmid

    #218164

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX335 HTT sgRNA-a

    Plasmid

    #87201

    Purpose

    pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    HTT (HTT Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX335 HTT sgRNA-b

    Plasmid

    #87200

    Purpose

    pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    HTT (HTT Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFGC-I2Cas9

    Plasmid

    #173158

    Purpose

    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Inserts

    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Plant

    Mutation

    Contains a D169N mutation with no visible effect …

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgEGFR(A)

    Plasmid

    #85564

    Purpose

    Expresses sgRNA targeting human EGFR around codon T790

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    epidermal growth factor receptor (EGFR Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid

    #129412

    Purpose

    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgACSL3.C (ACSL3 Human)
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235)

    Plasmid

    #160646

    Purpose

    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S, 35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgALK(Chr. Inv.)

    Plasmid

    #85569

    Purpose

    Expresses sgRNA targeting human ALK between exon 20 and 21

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

20. sgEGFR(B)

    Plasmid

    #85565

    Purpose

    Expresses sgRNA targeting human EGFR around codon T790

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    epidermal growth factor receptor (EGFR Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only