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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,632 results for: sms

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Showing: 5781 - 5800 of 10632 results

  2. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  3. pINTO-N3::hTET3 HxD

    Plasmid
    #136365
    Purpose
    Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells
    Depositor
    Roberto Bonasio
    Publication
    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET3 (TET3 Human)
    Tags
    2xStrepTagII, FLAG, and HA
    Expression
    Mammalian
    Mutation
    HxD (H1077Y, D1079A)
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136365-plasmid-map-sequence-id-271993

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  4. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  9. DCT.VV.Orf212

    Plasmid
    #12900
    Purpose
    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
    Depositor
    Jack Bennink, Jon Yewdell
    Publication
    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VV.Orf212
    Expression
    Mammalian
    Available since
    Dec. 29, 2006
    Availability
    Academic Institutions and Nonprofits only

  13. DCT.VV.Orf76

    Plasmid
    #12764
    Purpose
    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
    Depositor
    Jack Bennink, Jon Yewdell
    Publication
    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VV.Orf76
    Expression
    Mammalian
    Available since
    Dec. 6, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Publication
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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Showing: 5781 - 5800 of 10632 results