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pAM-PAT-ProGL2 GW

Plasmid

#80689

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Weinl et al Plant Cell. 2005 Jun;17(6):1704-22. Epub

Type

Empty backbone

Expression

Plant

Promoter

ProGL2

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo small T H42Q

Plasmid

#9065

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pIS1 IMP-1 rc

Plasmid

#21643

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pFbx15(-640/-527)-TK-Luc

Plasmid

#13413

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
ath5:GFP-DCX

Plasmid

#105959

Purpose

transgenesis, doublecortin DCX

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-PIM2mut1

Plasmid

#51849

Purpose

promoter region of PIM2 lacking the forkhead binding site #2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-FRB-Venus-173-N

Plasmid

#87615

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-Venus173-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFbx15(-701/+114)-Luc

Plasmid

#13409

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S274A

Plasmid

#44107

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S274A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 274 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pFUS_A4B

Plasmid

#43854

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-D24Y

Plasmid

#30374

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-D24Y (PTEN Human)

Expression

Mammalian

Mutation

D24Y

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pSR21

Plasmid

#69156

Purpose

read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-mEKLF

Plasmid

#67838

Purpose

Expression of murine EKLF (nucleotides 71-1215) driven by SV40 promoter

Depositor

James Bieker

Article

Miller et al Mol Cell Biol. 1993 May;13(5):2776-86.

Insert

EKLF (Klf1 Mouse)

Tags

none

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
Syn3D146

Plasmid

#14677

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 1-146…

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only
pNB788

Plasmid

#60165

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pWB-TRAF2 S11A

Plasmid

#44134

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Retroviral

Tags

Flag

Mutation

Serine 11 to Alanine

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCSP1-HA-xEcto

Plasmid

#20900

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (trim33 Frog)

Tags

HA

Expression

Mammalian

Mutation

contains full unsequenced 3' UTR

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only