We narrowed to 14,002 results for: flag

1. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127318

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

   Plasmid

   #32460

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   S129D, S133D, S137D

   Promoter

   human PGK

   Available since

   Feb. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

   Plasmid

   #73618

   Purpose

   Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   Venus
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A

   Promoter

   ADH1

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

   Plasmid

   #47430

   Depositor

   Peter McPherson

   Article

   Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

   Insert

   Clavesin1 (CLVS1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169194

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   3xFlag-His6

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

   Plasmid

   #90011

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Cdc25C S216A
   NBirA*-14-3-3e
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Ser 216 to Ala

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 FLAG mTOR 1968-2549 S2035T

   Plasmid

   #23000

   Depositor

   Jing Huang

   Article

   Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2035T aa 1968-2549 only

   Promoter

   Available since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

   Plasmid

   #112762

   Purpose

   express GFP-4EBP1-Rheb15(CAAX mutation)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Rheb15(CAAX mutant)

   Expression

   Mutation

   CAAX mutation to prevent lipid localization

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)

   Plasmid

   #47431

   Depositor

   Peter McPherson

   Article

   Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

   Insert

   Clavesin2 (CLVS2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCMV10/3xflag-Clock L57E/L113E/W284A

    Plasmid

    #47343

    Purpose

    triple mutation used for co-IP

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L57E/L113E/W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRK-flag-USP19 494-1216 (USP)

    Plasmid

    #78588

    Purpose

    Expression flag-USP19 494-1216 (USP) in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 494-1216 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-493 and mutated 1216 residue…

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5-Flag-EPLIN beta delta C term

    Plasmid

    #40931

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN beta (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminus deleted

    Promoter

    Available since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

    Plasmid

    #166796

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    KK93EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

    Plasmid

    #166800

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    E597R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

    Plasmid

    #49064

    Purpose

    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

    Plasmid

    #39859

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    C11orf51 (ANAPC15 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

    Plasmid

    #72608

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine37 to alanine

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pFLAG-4EHP(K121/130/134/222R)

    Plasmid

    #17353

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    4EHP (EIF4E2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K121/130/134/222R

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

19. mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

    Plasmid

    #73269

    Purpose

    mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCDNA3-N3FLAG-MYB-TAD(251-327)

    Plasmid

    #105611

    Purpose

    transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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