Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,276 results for: nes

Clear keywords
Showing: 5801 - 5820 of 16276 results

  7. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

    Enlarge

  8. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid
    #188072
    Purpose
    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.
    Depositor
    Kristen Verhey
    Publication
    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e
    Insert
    rat KIF5C (Kif5c Rat)
    Tags
    Flag and Halo
    Expression
    Mammalian
    Mutation
    aa1-560, deletion of aa2-6
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    188072-plasmid-map-sequence-id-368400

    Enlarge

  10. pEGFP-MyosinVb-Ctail

    Plasmid
    #110170
    Purpose
    Dominant negative effect by competing with MyosinVb C-terminal tail interactions
    Depositor
    Jose Esteban
    Publication
    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Myosin Vb (Myo5b Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    amino acids 1262-1670
    Promoter
    CMV
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    110170-plasmid-map-sequence-id-210548

    Enlarge

  15. AD-003

    Plasmid
    #25502
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AD-003 (NTMT1 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

    Enlarge

  18. pD3sE_SC12

    Plasmid
    #219720
    Purpose
    DV3 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Publication
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV3 sE SC12
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219720-plasmid-map-sequence-id-433924

    Enlarge

Showing: 5801 - 5820 of 16276 results