We narrowed to 45,523 results for: cha

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pNES-EGFP-C1-flex-PABD-Spo20

Plasmid

#220081

Purpose

Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

Spo20 (SPO20 Budding Yeast)

Tags

X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

Expression

Mammalian

Mutation

amino acids 51-91

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Etv6

Plasmid

#131599

Purpose

expression of mouse Etv6 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Etv6 (Etv6 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_WiChR_TS_mScarlet_ER

Plasmid

#205997

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Has Service

AAV9

Insert

Wobblia Inhibitory Channelrhodopsin

Use

AAV

Tags

TS, mScarlet, ER

Promoter

hSyn

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr_LF(C199S)

Plasmid

#216114

Purpose

Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

oROS-Gr_LF(C199S)

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 DN

Plasmid

#13051

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); aspara…

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pTW040

Plasmid

#64769

Purpose

For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Promoter

TDH3

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6B (full-length, aa 1-293)

Plasmid

#61418

Purpose

Expresses human OTUD6B (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP

Plasmid

#139283

Purpose

Express hChR2(H134R)-P2A-EYFP in a Cre-dependent manner

Depositor

Mingshan Xue

Article

Chen et al Elife. 2020 Feb 19;9. pii: 48705. doi: 1

Insert

hChR2(H134R)-P2A-EYFP

Use

AAV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

Plasmid

#176742

Purpose

Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKIIa iChloC 2A tDimer

Plasmid

#66709

Purpose

Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

CaMKIIa

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176741

Purpose

Expresses membrane bound biotin ligase, BioID2, in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Ezr-BioID2-HA

Plasmid

#176744

Purpose

Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Ezr-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-pcDNA3.1

Plasmid

#85760

Purpose

mammalian expression of full length HA tagged USPL1

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length (USPL1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSS146

Plasmid

#64770

Purpose

For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Promoter

TDH4

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (full-length, aa 1-481)

Plasmid

#61405

Purpose

Expresses human OTUD1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

Plasmid

#186835

Purpose

Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 513/515/516 to Alanine

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan-ADE2

Plasmid

#232105

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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