Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,036 results for: SIM

Showing: 5841 - 5860 of 7036 results

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

Plasmid

#114613

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TARDBP)

Plasmid

#109323

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TARDBP Human)

Use

AAV

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650A

Plasmid

#171252

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650D

Plasmid

#171253

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

Plasmid

#114611

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

Plasmid

#114617

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C

Plasmid

#114622

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 43 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C

Plasmid

#114623

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q

Plasmid

#114627

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

Plasmid

#114631

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 49 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA mutant

Plasmid

#244882

Purpose

In vitro transcription of mutant human FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

GRCh38, chr2:70300842G>A

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA WT

Plasmid

#244883

Purpose

In vitro transcription of wild-type FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-mutant-3xFLAG-hygro

Plasmid

#244879

Purpose

Overexpression of mutant human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

GRCh38, chr2:70300842G>A

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg2

Plasmid

#244864

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg1

Plasmid

#244863

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg2

Plasmid

#244852

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg1

Plasmid

#244851

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)

Plasmid

#239353

Purpose

Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA(mut 8356-8370)

Expression

Mammalian

Mutation

MALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccacc…

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_RPP21 (pAVA3259)

Plasmid

#239327

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPP21

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPP21 (RPP21 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 5841 - 5860 of 7036 results

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