We narrowed to 18,384 results for: ids
-
Plasmid#25253PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertKIFC1:G361-C715 (KIFC1 Human)
UseBaculovirus expressionTagsHisMutationContains amino acids G361-C715PromoterpolyhedrinAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Myc-Foxo1 LAALL (pCMV5)
Plasmid#17548DepositorInsertFoxo1 LAALL (Foxo1 Mouse)
UseRNAiTagsMycExpressionMammalianMutationLAALL (K460A; E461A)PromoterCMVAvailable SinceOct. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
816 pSG5 HA PTEN delta89-138
Plasmid#10924DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG2-EGFP-C-IFT122
Plasmid#218737PurposeExpresses C-terminally EGFP-tagged IFT122 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-ngRNA+41_EF1a-puroR
Plasmid#207358PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 +41 ngRNA
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal-BE3
Plasmid#172409PurposeYeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoterDepositorInsertBE3
UseCRISPRExpressionYeastPromoterGALLAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_nat
Plasmid#184911PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRuby2-CrowdingLinker-Clover
Plasmid#171058PurposeExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.DepositorInsertCrowdedness-sensitive linker domain
TagsmClover2 and mRuby2ExpressionMammalianPromoterCMVAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)
Plasmid#160629PurposeTU for the expression of LbCas12aDepositorInsertAtUBQ10-:LbCpf1(Cas12a):Tnos
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterUBQ10Available SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-HDAC1-H141A-Hygro
Plasmid#104417Purposetransient expression of dCas9-dHDAC1 fusion proteinDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - mCherry - BirA*
Plasmid#131136PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-mCherry-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available SinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRMutationrs140428747Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C-donor
Plasmid#113824PurposeCRISPR donor plasmid to tag human transcription factor TFAP2C with GFPDepositorInsertTFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
UseCRISPRMutationrs6025024, hg38:20:56638660:TTT/-Available SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2s
Plasmid#118036PurposeGABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminusDepositorInsertGABRG2 (Gabrg2 Rat)
TagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQRRA-P2A-Puro
Plasmid#110851PurposeLentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)DepositorInsertCas9-VQRRA
UseLentiviralTagsFLAGMutationD1135V, R1335Q, T1337R and NLS sequence at the N-…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
Tags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only