Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,488 results for: Dos

Clear keywords
Showing: 5881 - 5900 of 13488 results

  1. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Publication
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

    Enlarge

  6. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

    Enlarge

  7. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid
    #105287
    Purpose
    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L
    Depositor
    Eugene Yeo
    Publication
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    UBAP2L (UBAP2L Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deleted amino acids 20-108
    Promoter
    TRE promoter, Tet ON
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    105287-plasmid-map-sequence-id-223527

    Enlarge

  9. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Publication
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

    Enlarge

  18. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61362-plasmid-map-sequence-id-99796

    Enlarge

  20. Tmem98-sGFP2

    Plasmid
    #124503
    Purpose
    Expresses sGFP2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)
    Depositor
    Renee van Amerongen
    Publication
    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tmem98 (Tmem98 Mouse)
    Tags
    sGFP2
    Expression
    Mammalian
    Mutation
    stop codon was removed to allow fusion with sGFP2…
    Promoter
    CMV
    Available since
    May 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    124503-plasmid-map-sequence-id-254376

    Enlarge

Showing: 5881 - 5900 of 13488 results